FITC Anti-Rotavirus antibody (ab31435)

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Key features and details

  • FITC Goat polyclonal to Rotavirus
  • Suitable for: ICC/IF
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-Rotavirus antibody
    See all Rotavirus primary antibodies

  • Description

    FITC Goat polyclonal to Rotavirus

  • Host species

    Goat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Tissue/ cell preparation: Bovine rotavirus (whole virus).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Ion Exchange Chromatography

  • Purification notes

    Ammonium sulfate fractionation and ion-exchange chromatography.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab31435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250. Acetone fixed.

Target

  • Relevance

    Rotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.

  • Database links

    • Alternative names

      • Major inner capsid protein VP6 antibody
      • NSP4 antibody
      • VP6 antibody

    References (2)

    Publishing research using ab31435? Please let us know so that we can cite the reference in this datasheet.

    ab31435 has been referenced in 2 publications.

    • Ryabchevskaya EM  et al. A Recombinant Rotavirus Antigen Based on the Coat Protein of Alternanthera Mosaic Virus. Mol Biol 54:243-248 (2020). PubMed: 32431461
    • Buccigrossi V  et al. Mechanisms of antidiarrhoeal effects by diosmectite in human intestinal cells. Gut Pathog 9:23 (2017). PubMed: 28450899

    Customer reviews and Q&As

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    Question

    Immunogen for ab31435. Is it the VP4, VP6 etc domain?

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    Answer

    Thank you for contacting us.

    The immunogen for this antibody is Nebraska Calf Diarrhea Virus (whole virus).



    The antibody recognizes rotavirus and is specific for ICPs and late structural (virion) antigens. No further epitope information has been determined.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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