FITC Anti-Semaphorin 7a antibody [MEM-150] (ab26012)
![Flow Cytometry - FITC Anti-Semaphorin 7a antibody [MEM-150] (ab26012)](https://www.abcam.com/ps/products/26/ab26012/Images/ab26012-36509-anti-semaphorin-7a-antibody-mem-150-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-Semaphorin 7a antibody [MEM-150] (ab26012)")
Key features and details
- FITC Mouse monoclonal [MEM-150] to Semaphorin 7a
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Overview
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Product name
FITC Anti-Semaphorin 7a antibody [MEM-150]
See all Semaphorin 7a primary antibodies -
Description
FITC Mouse monoclonal [MEM-150] to Semaphorin 7a -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
T cell line HPB-ALL. (Human)
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General notes
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-150 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab26012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 20µl for 106 cells. Use 20 µl per 100 µl of whole blood. ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
Tissue specificity
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
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Database links
- Entrez Gene: 8482 Human
- Omim: 607961 Human
- SwissProt: O75326 Human
- Unigene: 24640 Human
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Alternative names
- CD 108 antibody
- CD108 antibody
- CD108 antigen antibody
see all
Images
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Flow Cytometry - FITC Anti-Semaphorin 7a antibody [MEM-150] (ab26012)Overlay histogram showing Jurkat cells stained with ab26012 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26012, 1.2µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgGM FITC (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References (0)
ab26012 has not yet been referenced specifically in any publications.
