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  1. Link

    fitc-staphylococcus-aureus-antibody-ab68950.pdf

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Tags & Cell Markers Fusion / Marker Proteins Protein A
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FITC Anti-Staphylococcus aureus antibody (ab68950)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Abpromise

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Find out more.

Key features and details

  • FITC Rabbit polyclonal to Staphylococcus aureus
  • Suitable for: ICC/IF, IHC-Fr
  • Reacts with: Staphylococcus aureus
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-Staphylococcus aureus antibody
    See all Staphylococcus aureus primary antibodies
  • Description

    FITC Rabbit polyclonal to Staphylococcus aureus
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Staphylococcus aureus
  • Immunogen

    Soluble and structural antigens of Staphylococcus aureus whole bacterium; ATCC #27660.

  • General notes


    This antibody reacts with Staphylococcus aureus in bacterial and infected tissue samples.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Primary antibody notes

    This antibody reacts with Staphylococcus aureus in bacterial and infected tissue samples.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Protein A
    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Staphylococcus
    • Microbiology
    • Toxin
    • Bacterial Toxin
    • Staphylococcus Toxin

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

Our Abpromise guarantee covers the use of ab68950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10.
IHC-Fr 1/10.

Target

  • Relevance

    S.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Secreted; cell wall.
  • Database links

    • Entrez Gene: 3919448 Staphylococcus aureus
    • SwissProt: P02976 Staphylococcus aureus
    • Alternative names

      • IgG binding protein A antibody
      • Immunoglobulin G binding protein A antibody
      • SPA antibody

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab68950? Please let us know so that we can cite the reference in this datasheet.

    ab68950 has been referenced in 1 publication.

    • Becerra SC  et al. An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue. BMC Res Notes 9:216 (2016). PubMed: 27071769

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Good afternoon.
    Do you have any answer?

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2012

    Answer

    Thank you for contacting us.

    Please find below the information I managed to collect by contacting the sources of the Anti-Staphylococcus aureusand the Anti-Streptococcus agalactiaeantibodies we have in our catalog :

    Anti-Staphylococcus aureus:
    https://www.abcam.com/ab23568 shows no reactivity to any streptococcus sp., mycoplasma sp. or corynebacteria sp.

    Unfortunately, ab35192 has not been specifically tested against streptococcus so we are unable to guarantee it won’t react to S. agalactiae.

    ab68950, ab68952, ab68954: These Staphylococcus aureus polyclonal antibodies are not absorbed for related microorganisms and so therefore could cross react with related bacteria such as Staph. Epidermidis.

    MLS : Staphylococcus aureus: We do not have an antibody that is specific to Staph. aureus but does not have crossreactivity to Staph. epidermidis.

    Anti-Streptococcus agalactiae:
    https://www.abcam.com/ab21126 has weak crossreactivity with Strep. dysgalactiae and does not cross-react with Strep. uberis, Strep. bovis, Strep. faecalis, Staph. aureus, Mycoplasma bovis, Mycoplasma californicum, Mycoplasma bovigenitalium, E. coli or Corynebacterium bovis.

    I hope this information is helpful to you. I am still waiting for more information about ab128711 (Anti-Streptococcus agalactiae antibody [224/46]).
    Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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