RecombinantRabMAb

Recombinant FITC Anti-STAT6 antibody [YE361] (ab223918)

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Flow Cytometry - FITC Anti-STAT6 antibody [YE361] (ab223918)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-STAT6 antibody [YE361] (ab223918)
  • FITC Anti-STAT6 antibody [YE361] (ab223918)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • FITC Rabbit monoclonal [YE361] to STAT6
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm

Overview

  • Product name

    FITC Anti-STAT6 antibody [YE361]
    See all STAT6 primary antibodies

  • Description

    FITC Rabbit monoclonal [YE361] to STAT6

  • Host species

    Rabbit

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    The antibody does not cross-react with other Stat family members.

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P42226

  • Positive control

    • ICC/IF: NIH3T3 cells. Flow Cyt: NIH3T3 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab223918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in NIH3T3 cells fixed with 100% methanol (5 min)
Flow Cyt 1/50.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

  • Database links

    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-STAT6 antibody [YE361] (ab223918)
      Flow Cytometry - FITC Anti-STAT6 antibody [YE361] (ab223918)

      Overlay histogram showing NIH3T3 cells stained with ab223918 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223918, 1/50 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-STAT6 antibody [YE361] (ab223918)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-STAT6 antibody [YE361] (ab223918)

      ab223918 staining STAT6 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223918 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • FITC Anti-STAT6 antibody [YE361] (ab223918)
      FITC Anti-STAT6 antibody [YE361] (ab223918)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab223918? Please let us know so that we can cite the reference in this datasheet.

    ab223918 has not yet been referenced specifically in any publications.

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