Recombinant FITC Anti-STAT6 antibody [YE361] (ab223918)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [YE361] to STAT6
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Related conjugates and formulations
Overview
-
Product name
FITC Anti-STAT6 antibody [YE361]
See all STAT6 primary antibodies -
Description
FITC Rabbit monoclonal [YE361] to STAT6 -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
The antibody does not cross-react with other Stat family members. -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: NIH3T3 cells. Flow Cyt (intra): NIH3T3 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE361 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 488 Anti-STAT6 antibody [YE361] (ab196478)
- HRP Anti-STAT6 antibody [YE361] (ab196480)
- Alexa Fluor® 647 Anti-STAT6 antibody [YE361] (ab196928)
- Alexa Fluor® 594 Anti-STAT6 antibody [YE361] (ab207012)
- Alexa Fluor® 405 Anti-STAT6 antibody [YE361] (ab207013)
- Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (ab207014)
- Alexa Fluor® 568 Anti-STAT6 antibody [YE361] (ab207015)
- Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)
- PE Anti-STAT6 antibody [YE361] (ab223917)
- APC Anti-STAT6 antibody [YE361] (ab223919)
- Anti-STAT6 antibody [YE361] (ab32520)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/1000.
This product gave a positive signal in NIH3T3 cells fixed with 100% methanol (5 min) |
|
Flow Cyt (Intra) |
1/50.
|
Notes |
---|
ICC/IF
1/1000. This product gave a positive signal in NIH3T3 cells fixed with 100% methanol (5 min) |
Flow Cyt (Intra)
1/50. |
Target
-
Function
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
-
Database links
- Entrez Gene: 6778 Human
- Entrez Gene: 20852 Mouse
- Omim: 601512 Human
- SwissProt: P42226 Human
- SwissProt: P52633 Mouse
- Unigene: 524518 Human
-
Alternative names
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
Images
-
Overlay histogram showing NIH3T3 cells stained with ab223918 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223918, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
-
ab223918 staining STAT6 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223918 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab223918 has not yet been referenced specifically in any publications.