FITC Anti-TCR alpha + TCR beta antibody [H57-597] (ab25010)
![Flow Cytometry - FITC Anti-TCR alpha + TCR beta antibody [H57-597] (ab25010)](https://www.abcam.com/ps/products/25/ab25010/Images/ab25010-276644-anti-tcr-alpha-tcr-beta-antibody-h57-597-fitc-flow-cytometry.png "Flow Cytometry - FITC Anti-TCR alpha + TCR beta antibody [H57-597] (ab25010)")
Key features and details
- FITC Armenian hamster monoclonal [H57-597] to TCR alpha + TCR beta
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-TCR alpha + TCR beta antibody [H57-597]
See all TCR alpha + TCR beta primary antibodies -
Description
FITC Armenian hamster monoclonal [H57-597] to TCR alpha + TCR beta -
Host species
Armenian hamster -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)
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General notes
ab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS -
Primary antibody notes
ab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells. -
Clonality
Monoclonal -
Clone number
H57-597 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab25010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Relevance
The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 21473 Mouse
- Entrez Gene: 21577 Mouse
- SwissProt: P01849 Mouse
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Alternative names
- FLJ22602 antibody
- MGC117436 antibody
- MGC22624 antibody
see all
Images
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Flow Cytometry - FITC Anti-TCR alpha + TCR beta antibody [H57-597] (ab25010)
Flow Cytometry analysis of C57Bl/6 splenocytes labeling TCR alpha + TCR beta with ab25010 at 0.25µg (solid line). Armenian hamster IgG (FITC) isotype control at 0.25µg (dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab25010 has been referenced in 1 publication.
- Kubo RT et al. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol 142:2736-42 (1989). PubMed: 2467936
