FITC Anti-TIL/TLR1 antibody [GD2.F4] (ab59702)
![Flow Cytometry - FITC Anti-TIL/TLR1 antibody [GD2.F4] (ab59702)](https://www.abcam.com/ps/products/59/ab59702/Images/ab59702-73615-anti-til-tlr1-antibody-gd2f4-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-TIL/TLR1 antibody [GD2.F4] (ab59702)")
Key features and details
- FITC Mouse monoclonal [GD2.F4] to TIL/TLR1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
-
Product name
FITC Anti-TIL/TLR1 antibody [GD2.F4]
See all TIL/TLR1 primary antibodies -
Description
FITC Mouse monoclonal [GD2.F4] to TIL/TLR1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody reacts with human TIL/TLR1. It does not recognize TLR2, TLR4 or TLR4 in association with MD2. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human TIL/TLR1.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2µm filtered solution. -
Clonality
Monoclonal -
Clone number
GD2.F4 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab59702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Cellular localization
Cell membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 7096 Human
- Omim: 601194 Human
- SwissProt: Q15399 Human
- Unigene: 621817 Human
- Unigene: 654532 Human
-
Alternative names
- CD281 antibody
- CD281 antigen antibody
- KIAA0012 antibody
see all
Images
-
Flow Cytometry - FITC Anti-TIL/TLR1 antibody [GD2.F4] (ab59702)
Overlay histogram showing TIL/THP1 cells stained with ab59702 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59702, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Datasheets and documents
References (0)
ab59702 has not yet been referenced specifically in any publications.
