FITC Anti-TLR4 antibody [HTA125] (ab8378)

Submit a review Q&A (1)References (4)
Flow Cytometry - FITC Anti-TLR4 antibody [HTA125] (ab8378)

    Key features and details

    • FITC Mouse monoclonal [HTA125] to TLR4
    • Suitable for: Flow Cyt
    • Reacts with: Guinea pig, Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-TLR4 antibody [HTA125]
      See all TLR4 primary antibodies

    • Description

      FITC Mouse monoclonal [HTA125] to TLR4

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Guinea pig, Human

    • Immunogen

      Ba/F3 cell line expressing TLR4

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab8378 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Use 10 µl of this concentration to label 106 cells or 100µl whole blood.

     

     

     

    ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

    • Tissue specificity

      Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

    • Involvement in disease

      Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 18 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.

    • Domain

      The TIR domain mediates interaction with NOX4.

    • Post-translational
      modifications

      N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARMD10 antibody
        • CD284 antibody
        • CD284 antigen antibody
        • Homolog of Drosophila toll antibody
        • hToll antibody
        • TLR 4 antibody
        • TLR4 antibody
        • TLR4_HUMAN antibody
        • TOLL antibody
        • Toll like receptor 4 antibody
        • Toll-like receptor 4 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-TLR4 antibody [HTA125] (ab8378)
        Flow Cytometry - FITC Anti-TLR4 antibody [HTA125] (ab8378)
        ab8378 staining TLR4 (FITC) in human peripheral blood monocytes by Flow Cytometry analysis.

      References (4)

      Publishing research using ab8378? Please let us know so that we can cite the reference in this datasheet.

      ab8378 has been referenced in 4 publications.

      • Ye Y  et al. Meisoindigo Protects Against Focal Cerebral Ischemia-Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation and Regulating Microglia/Macrophage Polarization via TLR4/NF-?B Signaling Pathway. Front Cell Neurosci 13:553 (2019). PubMed: 31920554
      • Hou Y  et al. Up-regulation of DcR3 in microbial toxins-stimulated HUVECs involves NF-?B signalling. BMC Biochem 19:13 (2018). PubMed: 30587127
      • Velot E  et al. Activation of the adenosine-A3 receptor stimulates matrix metalloproteinase-9 secretion by macrophages. Cardiovasc Res : (2008). PubMed: 18653544
      • Falcone S  et al. Activation of acid sphingomyelinase and its inhibition by the nitric oxide/cyclic guanosine 3',5'-monophosphate pathway: key events in Escherichia coli-elicited apoptosis of dendritic cells. J Immunol 173:4452-63 (2004). PubMed: 15383576

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      In my project I search for a possibility to show that different mouse cells express TLR 2, 4 and 9. You offer FITC antibodies recognizing anti-human TLR . In the data sheet you say that you didn´t test for cross-reactivity. Is this still correct or did you test it for mouse cross-reactivity? Are there any antibodies specific for mouse TLRs ? Otherwise, if you would be so kind to give me a probe of this antibody, I would test it for cross-ractivity with my isolated mouse cells and give you a feedback for the data sheet.

      Read More
      Answer

      We do not know whether this antibody will cross react with mouse tissue. We do not have any trial samples for testing purposes, but would be interested to know whether this antibody cross reacts with mouse. If you decide to purchase this antibody, please let us know whether it cross reacts with mouse and we'll offer you a EUR 25.00 Amazon gift voucher as a reward! (Please see the reviews link on the datasheet for more details). This is the only FITC-labled TLR-4 antibody that we currently have in stock.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com