FITC Anti-TLR9 antibody [5G5] (ab58864)

Submit a review Q&A (8)References (4)
Flow Cytometry - FITC Anti-TLR9 antibody [5G5] (ab58864)

    Key features and details

    • FITC Mouse monoclonal [5G5] to TLR9
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Dog, Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-TLR9 antibody [5G5]
      See all TLR9 primary antibodies

    • Description

      FITC Mouse monoclonal [5G5] to TLR9

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse, Dog, Human

    • Immunogen

      Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc

    • Positive control

      • RAW macrophages stimulated with IFN-gamma

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab58864 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/10 - 1/50.

    ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

    • Tissue specificity

      Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 26 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.

    • Cellular localization

      Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 289 antibody
        • CD289 antibody
        • TLR 9 antibody
        • TLR9 antibody
        • TLR9_HUMAN antibody
        • Toll like receptor 9 antibody
        • Toll like receptor 9 isoform A precursor antibody
        • Toll like receptor 9 isoform B antibody
        • Toll-like receptor 9 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-TLR9 antibody [5G5] (ab58864)
        Flow Cytometry - FITC Anti-TLR9 antibody [5G5] (ab58864)
        THP1 cells were incubated with an IgG2a isotype control (A) or a-TLR9 5G5 mAb (B). Cells (140000) were permeabilized with saponin and stained with 0.4 µg 5G5.

      References (4)

      Publishing research using ab58864? Please let us know so that we can cite the reference in this datasheet.

      ab58864 has been referenced in 4 publications.

      • Frelinger AL  et al. Tunable activation of therapeutic platelet-rich plasma by pulse electric field: Differential effects on clot formation, growth factor release, and platelet morphology. PLoS One 13:e0203557 (2018). PubMed: 30256831
      • Dias AA  et al. DNA Sensing via TLR-9 Constitutes a Major Innate Immunity Pathway Activated during Erythema Nodosum Leprosum. J Immunol 197:1905-13 (2016). PubMed: 27474073
      • Ray A  et al. A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib. Leukemia N/A:N/A (2014). Human . PubMed: 24476765
      • Boura JS  et al. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev 2014:N/A (2014). Flow Cyt ; Human . PubMed: 25279386

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-8 of 8 Abreviews or Q&A

      Question

      Bonjour,
      Merci pour votre réponse.
      J'aimerais tester le produit alternatif que vous me proposer (ab134369) pour m'assurer que avec ce clone, je ne peux pas obtenir un autre résultat.
      Merci d'avance
      Bien à vous

      Read More
      Answer

      Merci pour votre réponse.
      Comme convenu j'ai mis en place l'envoi d'une unité de ab134369 en remplacement du ab58864. Le numéro de commande de remplacement gratuit de ce produit est : ******.
      Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.
      N'hésitez pas à nous contacter lors d'une prochaine occasion.

      Read More

      Question

      Hello,
      I recently used your antibodyanti-TLR9 (5G5) (FITC) (ab58864)and it appears that this antibody seems not to be specific for the TLR9 protein.
      Please find enclosed my explanations and questions.
      Kind regards

      Read More
      Answer

      Merci de nous avoir contactés.
      Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu.
      Merci pour le partage de vos résultats et de votre protocole, ces informations sont très importantes pour nous car elles nous permettent d'investiguer la source du problème rencontré avec cet anticorps et de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits.
      Je pense que l'anticorps aurait du fonctionner, j'aimerais donc vous proposer un remboursement, ou un avoir, ou un anticorps de remplacement.
      Pour l'anticorps de remplacement, je peux vous proposer un produit alternatif, le ab134369 (www.abcam.com/ab134369). Alternativement je peux vous envoyer un autre tube de ab58864 mais celui-ci sera du même lot que celui que vous avez reçu et qui n'a pas fonctionné.
      Merci de m'indiquer la résolution qui vous conviendrait le mieux.

      Read More

      Question

      To whomever this may concern,
      I am interested in finding out the immunogen sequences for your antibodies Ab72362, Ab58864, and Ab16892 as they are not listed on your website. Do you have this information?

      Read More
      Answer

      Thank you for your inquiry.

      The products ab16892 and ab58864 are both from the same clone [5G5] and have the same immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.The immunogen for both is as described in the paper below:

      Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958

      Regarding ab72362, the immunogen is BALB/c mice immunized with Ba/F3 cells that stably expressed human Myc-tagged TLR6. The epitope has not been mapped.
      I hope this information helps. Please contact us with any other questions.

      Read More

      Question

      To whomever this may concern,

      I am writing to inquire about your following products Ab72362, Ab22046, Ab58864
      and Ab16892.
      We are planning on completing Flow Cytometry on bovine blood mononuclear cells
      in order to quantify the expression of TLRs 2, 4, 6 and 9. We have already found
      a bovine-specific antibody for TLR2, but are still looking for antibodies to the
      other TLRs.
      We are interested if you have tested and proved the cross reactivity of your
      products above for bovine. If you have, or if you have other products that
      would be suitable, please let us know. If you have not, we were hoping to get a
      small aliquot of these antibodies in order to test them for cross reactivity to
      bovine ourselves.

      Read More
      Answer

      Thank you very much for your interest in our products.

      To our knowledge,none of our TLR6 or TLR9 antibodies havebeen tested in bovine. Since we have over 90,000 products we cannot store multiple vial sizes for testing. Therefore, I can offer a discount off a future purchase if you buyanti-TLR6 and anti-TLR9antibodies now, test them inbovine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per antibody tested.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceedand let me know the catalogue number of the product/s youwould like to testin bovine. I will then send thediscount code/s.The codesmust be issued before purchasing the antibodies so please wait for my reply before ordering.

      2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

      3. Test it in bovine.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for bovine, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

      Read More

      Question

      Is the concentration of FITC in ab58864 40x as in Ab81197 ( IgG2a Isotype)?

      I checked the TLR9 antibody list. Is the Anti-TLR9 antibody [5G5] (ab16892) exactly same as ab58864 except FITC labeling? However, it does not react with human cells. Do you have Anti-TLR9 antibody [5G5] which reacts with human and non-labeling?

      thanks

      Read More
      Answer

      Thank you for your reply.

      Although the exact amount of FITC in ab58864 and ab81197 has not been determined, for the purposes of your calculations, you can assume that it is 40x greater.

      Both ab16892 and ab58864, are the same clone of antibody, but the ab58864 has a FITC label. You could also use ab12121:

      https://www.abcam.com/TLR9-antibody-26C593-ab12121.html

      If there is anything else I can help you with, please let me know.

      Read More

      Question

      Hi, Anti-TLR9 antibody [5G5] (FITC) (ab58864) worked very good in our expt. Now question is about FITCIgG2a isotype control (ab81197). What is the concentration? What is the dilution if we use it as control for ab58864. Besides, can we purchase the same anti-TLR9 antibody withoutFITC labeling from you? thanks

      Read More
      Answer

      Thank you for contacting us.

      Ab81197 Mouse IgG2a (FITC) - Isotype control is supplied in a500 µl size at 4mg/ml. To correctly use this control you will need to use this at the same concentration as yourab58864 Anti-TLR9 antibody [5G5] (FITC) which is supplied at 0.1mg/ml. You will therefore need 40x less of the isotype control than you use for the TLR9 antibody.

      We do carry a number of alternative TLR9 antibodies many of which are guaranteed to work in Flow Cytometry. The entire list may be found at the following link:

      https://www.abcam.com/index.html?t=73449&pt=1

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

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      Question

      What is the epitope sequence of ab58864?

      Read More
      Answer

      Thank you for your inquiry.

      Unfortunately the epitope for this antibody is not determined.

      The following article provides more information regarding the immunogen used: Ahmad-Nejad, P et al; Eur J Immunol 2002, 32: 1958

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

      Read More

      Question

      dear sir,
      I need to buy an anti TLR9 antibody for immunofluorescence staining of murine cells.
      I've read that you have an anti mouse TLR9 in your catalogue (clone 5G5).
      Is possible to have some reference in which this antibody was used, or a fluorescence microscopy image obtained using this antibody, that validate the use of this antibody for immunofluorescence in mice?
      Thanks in advance

      Read More
      Answer

      Thank you for contacting us.

      Unfortunately, all the references and images available are already on the datasheet. We are continuously updating our datasheets, and as soon as we gather further characterisation details about it, we will share it on the Website.

      We also encourage our antibody’s users to send us the images obtained with the antibodies and earn Abpoints in compensation: https://www.abcam.com/abpoints.

      However, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

      To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com