FITC Anti-TLR9 antibody [ABM4D70] (ab210925)
![Flow Cytometry - FITC Anti-TLR9 antibody [ABM4D70] (ab210925)](https://www.abcam.com/ps/products/210/ab210925/Images/ab210925-258429-anti-tlr9-antibody-abm4d70-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-TLR9 antibody [ABM4D70] (ab210925)")
Key features and details
- FITC Rat monoclonal [ABM4D70] to TLR9
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
FITC Anti-TLR9 antibody [ABM4D70]
See all TLR9 primary antibodies -
Description
FITC Rat monoclonal [ABM4D70] to TLR9 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse TLR9 aa 600-900.
Database link: Q9EQU3 -
Positive control
- Mouse splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM4D70 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.25µg for 106 cells.
|
| Notes |
|---|
|
Flow Cyt
Use 0.25µg for 106 cells. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 81897 Mouse
- SwissProt: Q9EQU3 Mouse
- Unigene: 44889 Mouse
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Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Images
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Flow Cytometry - FITC Anti-TLR9 antibody [ABM4D70] (ab210925)
Flow cytometric analysis of mouse splenocytes labeling TLR9 with ab210925 at 0.25 µg/106 cells (red) compared with negative control cells (green). FITC-conjugated secondary antibodies were used for the analysis.
Datasheets and documents
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Datasheet download
References (0)
ab210925 has not yet been referenced specifically in any publications.
