Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EPR20972] to TNF alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Product nameFITC Anti-TNF alpha antibody [EPR20972]
See all TNF alpha primary antibodies
DescriptionFITC Rabbit monoclonal [EPR20972] to TNF alpha
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse TNF alpha aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P06804
- Flow Cyt: LPS and BFA treated Raw264.7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab237356 in the following tested applications.
The cellular localisation of this product has been verified in ICC/IF.
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
Overlay histogram showing Raw264.7 cells untreated (green line) and Raw264.7 cells treated with lipopolysaccharides (LPS), 100ng/ml, 7hours, with addition of brefeldin A (BFA), 1ug/ml, for the last 3 hours (red line) stained with ab237356. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237356, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) fitc (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237356 has not yet been referenced specifically in any publications.