FITC Anti-Transferrin Receptor antibody [YTA 74.4] (ab33996)
Key features and details
- FITC Rat monoclonal [YTA 74.4] to Transferrin Receptor
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-Transferrin Receptor antibody [YTA 74.4]
See all Transferrin Receptor primary antibodies -
Description
FITC Rat monoclonal [YTA 74.4] to Transferrin Receptor -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Species reactivity
Reacts with: Mouse -
Immunogen
Concanavilin A activated mouse spleen cells.
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General notes
ab33996 stains dividing cells, and can be used to distinguish resting from activated T cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified IgG prepared by ion exchange chromatography. -
Primary antibody notes
ab33996 stains dividing cells, and can be used to distinguish resting from activated T cells. -
Clonality
Monoclonal -
Clone number
YTA 74.4 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
Target
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Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 22042 Mouse
- SwissProt: Q62351 Mouse
- Unigene: 28683 Mouse
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Datasheets and documents
References (4)
ab33996 has been referenced in 4 publications.
- Hauser D et al. Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing. Nanomaterials (Basel) 8:N/A (2018). PubMed: 30562983
- Fagoonee S et al. Lack of plasma protein hemopexin dampens mercury-induced autoimmune response in mice. J Immunol 181:1937-47 (2008). Flow Cyt ; Mouse . PubMed: 18641331
- Lesley J et al. Expression of transferrin receptor on murine hematopoietic progenitors. Cell Immunol 83:14-25 (1984). PubMed: 6319033
- Trowbridge IS et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J Cell Physiol 112:403-10 (1982). PubMed: 6290505
