Recombinant FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab237472)
![Flow Cytometry - FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab237472)](https://www.abcam.com/ps/products/237/ab237472/Images/ab237472-339440-ab237472FLOW1.jpg "Flow Cytometry - FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab237472)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Overview
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Product name
FITC Anti-VAV1 (phospho Y174) antibody [EP510Y]
See all VAV1 primary antibodies -
Description
FITC Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174) -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VAV1 (phospho Y174). The exact sequence is proprietary.
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Positive control
- Flow Cyt: Jurkat cells treated with Pervandate (1mM, 30 min)
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP510Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab237472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
Tissue specificity
Widely expressed in hematopoietic cells but not in other cell types. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The DH domain is involved in interaction with CCPG1. -
Post-translational
modificationsPhosphorylated on tyrosine residues. - Information by UniProt
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Database links
- Entrez Gene: 7409 Human
- Omim: 164875 Human
- SwissProt: P15498 Human
- Unigene: 116237 Human
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Alternative names
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
Images
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Flow Cytometry - FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab237472)
Overlay histogram showing Jurkat cells untreated (green line) and Jurkat cells treated with Pervanadate, 1mM, 30 minutes, (red line) stained with ab237472. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237472, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.
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![FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab237472)](https://www.abcam.com/ps/products/237/ab237472/Images/ab237472-3-benefits-of-recombinant-antibodies.png)
FITC Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab237472)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab237472 has not yet been referenced specifically in any publications.
