FITC Anti-VCP antibody [Hs-14] (ab77238)
Key features and details
- FITC Mouse monoclonal [Hs-14] to VCP
- Reacts with: Mouse, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Overview
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Product name
FITC Anti-VCP antibody [Hs-14]
See all VCP primary antibodies -
Description
FITC Mouse monoclonal [Hs-14] to VCP -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus corresponding to Human VCP. Freshly ejaculated human sperm washed in PBS & extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and then used for immunization.
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Positive control
- Sperm cells.
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General notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 0.2% Tris buffered saline
0.2% (w/v) high-grade protease free BSA -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
Hs-14 -
Isotype
IgM -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A. -
Involvement in disease
Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia. -
Sequence similarities
Belongs to the AAA ATPase family. -
Post-translational
modificationsPhosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. - Information by UniProt
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Database links
- Entrez Gene: 7415 Human
- Entrez Gene: 269523 Mouse
- Omim: 601023 Human
- SwissProt: P55072 Human
- SwissProt: Q01853 Mouse
- Unigene: 529782 Human
- Unigene: 245976 Mouse
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Alternative names
- 15S Mg(2+) ATPase p97 subunit antibody
- 15S Mg(2+)-ATPase p97 subunit antibody
- ALS14 antibody
see all
References (2)
ab77238 has been referenced in 2 publications.
- Peknicova J et al. Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol 53:42-9 (2005). PubMed: 15667524
- Chládek D et al. [Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization] Ceska Gynekol 65:28-32 (2000). PubMed: 10750294
