FITC Anti-Vimentin antibody [RV202] (ab128507)
![Immunohistochemistry (Frozen sections) - FITC Anti-Vimentin antibody [RV202] (ab128507)](https://www.abcam.com/ps/products/128/ab128507/Images/ab128507-142281-anti-vimentin-antibody-rv202-fitc-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - FITC Anti-Vimentin antibody [RV202] (ab128507)")
Key features and details
- FITC Mouse monoclonal [RV202] to Vimentin
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Goat, Chicken, Hamster, Dog, Human, Pig, Xenopus laevis, Monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-Vimentin antibody [RV202]
See all Vimentin primary antibodies -
Description
FITC Mouse monoclonal [RV202] to Vimentin -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Goat, Chicken, Hamster, Dog, Human, Pig, Xenopus laevis, Monkey
Does not react with: Zebrafish -
Immunogen
Tissue, cells or virus corresponding to Cow Vimentin. The immunogen is cytoskeletal vimentin extract of calf lens.
Database link: P08670 -
Positive control
- IHC-Fr: Swine colon tissue. Flow Cyt: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RV202 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab128507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/10 - 1/200. | |
| Flow Cyt | 1/1000. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 420519 Chicken
- Entrez Gene: 477991 Dog
- Entrez Gene: 102182515 Goat
- Entrez Gene: 101824289 Hamster
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 100522394 Pig
- Entrez Gene: 81818 Rat
see all -
Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - FITC Anti-Vimentin antibody [RV202] (ab128507)
ab128507, at 1/200 dilution, staining Vimentin in a frozen section of Swine colon tissue by Immunohistochemistry.
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![Flow Cytometry - FITC Anti-Vimentin antibody [RV202] (ab128507)](https://www.abcam.com/ps/products/128/ab128507/Images/ab128507-199339-anti-vimentin-antibody-rv202-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-Vimentin antibody [RV202] (ab128507)Overlay histogram showing HeLa cells stained with ab128507 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128507, 1/1000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 1μg/1x106 cells) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
References (6)
ab128507 has been referenced in 6 publications.
- Chen L et al. Zebrafish Microenvironment Elevates EMT and CSC-Like Phenotype of Engrafted Prostate Cancer Cells. Cells 9:N/A (2020). PubMed: 32225005
- Zhang X et al. Effects of ZnT8 on epithelial-to-mesenchymal transition and tubulointerstitial fibrosis in diabetic kidney disease. Cell Death Dis 11:544 (2020). PubMed: 32681069
- Su L et al. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis. Sci Rep 9:5747 (2019). PubMed: 30952998
- Theodoraki MN et al. Plasma-derived Exosomes Reverse Epithelial-to-Mesenchymal Transition after Photodynamic Therapy of Patients with Head and Neck Cancer. Oncoscience 5:75-87 (2018). PubMed: 29854876
- Vishnyakova PA et al. Mitochondrial role in adaptive response to stress conditions in preeclampsia. Sci Rep 6:32410 (2016). PubMed: 27573305
- Yew KH et al. Epimorphin-Induced MET Sensitizes Ovarian Cancer Cells to Platinum. PLoS One 8:e72637 (2013). PubMed: 24039787
