FITC Anti-Vimentin antibody [RV202] (ab128507)
![Immunohistochemistry (Frozen sections) - FITC Anti-Vimentin antibody [RV202] (ab128507)](https://www.abcam.com/ps/products/128/ab128507/Images/ab128507-142281-anti-vimentin-antibody-rv202-fitc-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - FITC Anti-Vimentin antibody [RV202] (ab128507)")
Key features and details
- FITC Mouse monoclonal [RV202] to Vimentin
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human, Pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-Vimentin antibody [RV202]
See all Vimentin primary antibodies -
Description
FITC Mouse monoclonal [RV202] to Vimentin -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human, Pig
Does not react with: Zebrafish -
Immunogen
Full length native protein (purified) corresponding to Cow Vimentin. Cytoskeletal vimentin extract of calf lens.
Database link: P48616 -
Positive control
- IHC-Fr: Swine colon tissue. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RV202 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/10 - 1/200.
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| Flow Cyt |
1/1000.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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IHC-Fr
1/10 - 1/200. |
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Flow Cyt
1/1000. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7431 Human
- Entrez Gene: 100522394 Pig
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P02543 Pig
- Unigene: 455493 Human
- Unigene: 691131 Human
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Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - FITC Anti-Vimentin antibody [RV202] (ab128507)
ab128507, at 1/200 dilution, staining Vimentin in a frozen section of Swine colon tissue by Immunohistochemistry.
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![Flow Cytometry - FITC Anti-Vimentin antibody [RV202] (ab128507)](https://www.abcam.com/ps/products/128/ab128507/Images/ab128507-199339-anti-vimentin-antibody-rv202-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-Vimentin antibody [RV202] (ab128507)Overlay histogram showing HeLa cells stained with ab128507 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128507, 1/1000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 1μg/1x106 cells) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab128507 has been referenced in 9 publications.
- Pan P et al. Vimentin regulation of autophagy activation in lung fibroblasts in response to lipopolysaccharide exposure in vitro. Ann Transl Med 9:304 (2021). PubMed: 33708931
- Niu R et al. Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy. Aging (Albany NY) 13:8643-8664 (2021). PubMed: 33714952
- Nobakht Lahrood F et al. Generation of Transplantable Three-Dimensional Hepatic-Patch to Improve the Functionality of Hepatic Cells In Vitro and In Vivo. Stem Cells Dev 29:301-313 (2020). PubMed: 31856676
- Chen L et al. Zebrafish Microenvironment Elevates EMT and CSC-Like Phenotype of Engrafted Prostate Cancer Cells. Cells 9:N/A (2020). PubMed: 32225005
- Zhang X et al. Effects of ZnT8 on epithelial-to-mesenchymal transition and tubulointerstitial fibrosis in diabetic kidney disease. Cell Death Dis 11:544 (2020). PubMed: 32681069
- Su L et al. Role of vimentin in modulating immune cell apoptosis and inflammatory responses in sepsis. Sci Rep 9:5747 (2019). PubMed: 30952998
- Theodoraki MN et al. Plasma-derived Exosomes Reverse Epithelial-to-Mesenchymal Transition after Photodynamic Therapy of Patients with Head and Neck Cancer. Oncoscience 5:75-87 (2018). PubMed: 29854876
- Vishnyakova PA et al. Mitochondrial role in adaptive response to stress conditions in preeclampsia. Sci Rep 6:32410 (2016). PubMed: 27573305
- Yew KH et al. Epimorphin-Induced MET Sensitizes Ovarian Cancer Cells to Platinum. PLoS One 8:e72637 (2013). PubMed: 24039787