FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)
![Flow Cytometry - FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)](https://www.abcam.com/ps/products/150/ab150266/Images/ab150266-245645-anti-zo1-tight-junction-protein-antibody-mabcam-61357-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)")
Key features and details
- FITC Mouse monoclonal [mAbcam 61357] to ZO1 tight junction protein
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357]
See all ZO1 tight junction protein primary antibodies -
Description
FITC Mouse monoclonal [mAbcam 61357] to ZO1 tight junction protein -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to ZO1 tight junction protein aa 750-850 conjugated to keyhole limpet haemocyanin.
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Positive control
- Flow Cyt: MCF7 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 61357 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab150266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.1µg for 106 cells. |
Target
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Function
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. -
Tissue specificity
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain. -
Domain
The second PDZ domain mediates interaction with GJA12. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRJ. -
Cellular localization
Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact. - Information by UniProt
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Database links
- Entrez Gene: 7082 Human
- Omim: 601009 Human
- SwissProt: Q07157 Human
- Unigene: 510833 Human
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Alternative names
- Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
see all
Images
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Flow Cytometry - FITC Anti-ZO1 tight junction protein antibody [mAbcam 61357] (ab150266)
Overlay histogram showing MCF7 cells stained with ab150266 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150266, 0.1μg/1x106 cells) for 30 min at 22ºC.
Isotype control antibody (black line) was mouse IgG1 [ICIG1] FITC (ab91356) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
References (3)
ab150266 has been referenced in 3 publications.
- Liu L et al. Angiotensin II inhibits the protein expression of ZO-1 in vascular endothelial cells by downregulating VE-cadherin. Mol Med Rep 18:429-434 (2018). PubMed: 29749551
- Li Y et al. Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways. Biomed Res Int 2018:3242574 (2018). PubMed: 29850504
- Huang J et al. Bioinspired Anti-digestive Hydrogels Selected by a Simulated Gut Microfluidic Chip for Closing Gastrointestinal Fistula. iScience 8:40-48 (2018). PubMed: 30273911
