• Product name

    Anti-FIV p24 antibody [PAK3-2C1] (Biotin)
    See all FIV p24 primary antibodies
  • Description

    Mouse monoclonal [PAK3-2C1] to FIV p24 (Biotin)
  • Host species

  • Conjugation

  • Specificity

    Recognises feline immunodeficiency virus (FIV) p24 gag. Does not recognise Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Feline Immunodeficiency Virus.
  • Immunogen

    Virus producer molecular clone of FIV.

  • Epitope

    The specific binding epitope recognised by clone PAK3-2C1 is an immunodominant p24 epitope and it appears to be enclosed in a tight peptide coil during gag production and maturation.
  • General notes

    ab53265 recognises a different epitope to ab54075.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab53265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.

    Note: The addition of 0.3% Triton-X detergent is recommended to reveal this
    epitope for antibody binding.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. p24 (Capsid protein) - part of the gag polyprotein, forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
    • Alternative names

      • Capsid protein p24 antibody
      • Feline immunodeficiency virus antibody
      • Feline immunodeficiency virus capsid protein p24 antibody
      • FIV capsid protein p24 antibody


    ab53265 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Indeed I can confirm that the ab54075 (clone NM3-6A9) recognizes a different epitope to ab53265 (clone PAK3-2C1) and that therefore they should be able to be used together in a Sandwich ELISA. I can also confirm that the biotinylated ab53265 is best if used as detection antibody. The issue however I was investigating is the adjunction of the Triton. Indeed, the ab53265 needs Triton in order to work. This should be added prior to the secondary antibody incubation accordingly to our source. However, we unfortunately do not have information whether the ab54075 does still recognize the epitope when the protein is treated with Triton. I can therefore not ascertain if these antibodies will work together. However we to have the ab53621 in our catalogue, which will work in the presence of Triton. I can therefore recommend to use the ab53621 in conjunction with the ab53265 in order to perform a sandwich ELISA - the ab53621 as coating and the ab53265 as detection antibody, as it is biotinylated. Click here (or use the following: https://www.abcam.com/index.html?datasheet=53621).

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