Overview

  • Product name
    Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry)
  • Product overview

    Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.


    The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.

  • Notes

    The kit protocol is very simple:

    - incubate cells with Fixation Medium for 15 min

    - add PBS and spin down cells

    - add Permeabilization Medium and antibody to cell pellet

    - incubate for 15 min

    - wash cells with PBS and analyze

    The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).

  • Tested applications
    Suitable for: Flow Cytmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab185917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Images

  • Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.

Protocols

References

ab185917 has not yet been referenced specifically in any publications.

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