Product nameFix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry)
Fix & Perm / Cell Fixation and Permeabilization Kit ab185917 is used to improve the staining of intracellular markers for flow cytometry. It is used to fix cells in suspension and then to permeabilize the cell membranes. It can be used for both cytoplasmic, mitochondrial and nuclear markers, as well as markers in other cellular organelles.
The reagents allow antibodies access to intracellular structures and leave the morphological scatter characteristics of cells intact. The formulation reduces background staining and allows the simultaneous addition of the permeabilization medium and fluorochrome labeled antibodies.
The kit protocol is very simple:
- incubate cells with Fixation Medium for 15 min
- add PBS and spin down cells
- add Permeabilization Medium and antibody to cell pellet
- incubate for 15 min
- wash cells with PBS and analyze
The overwhelming majority of flow cytometry antibodies are suitable for use with this kit (a limited number are sensitive to the fixation step).
Tested applicationsSuitable for: Flow Cytmore details
Our Abpromise guarantee covers the use of ab185917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and Lysozyme (FITC).
Double labeling of a normal blood sample treated with ab185917, and immunostained for CD3 (PE) and MPO-C2 (FITC).
Double labeling of a normal blood sample treated with ab185917, and immunostained for Lactoferrin (PE) and MPO-C2 (FITC).
Forward and side scatter pattern of a normal blood sample treated with ab185917.
Immunostaining with anti MPO-FITC conjugate of undifferentiated leukemia cells of AML type.
Immunostaining with CD3-PE conjugate of surface CD3-negative undifferentiated leukemia cells of T-ALL type.
Immunostaining with CD22-PE conjugate of undifferentiated leukemia cells of B-ALL type.
Flow cytometric analysis of normal bone marrow (BM) after fixation and pemeabilization using Fix and Perm kit ab185917, followed by immunostaining for the intra-cellular antigens Lactoferrin and MPO.
Flow cytrometric analysis of bone marrow (BM) from a leukemia patient after fixation and pemeabilization using ab185917, followed by immunostaining for lactoferrin and MPO-C2.
ab185917 has not yet been referenced specifically in any publications.