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Abcam’s CytoPainter Fixable Cell Viability Assay Kit (Fluorometric – Green Ex 405 nm) (ab176739) is used to evaluate the viability of mammalian cells by flow cytometry.
The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/Em =408/512 nm excited with a violet laser at Ex=405 nm.
|DMSO||1 x 200µl|
|Tracking dye Violet 500||1 vial|
Our Abpromise guarantee covers the use of ab176739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
ab176739 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"