Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Product nameFixable Cell Viability Assay Kit (Fluorometric - Orange) - Cytopainter
See all Cell Viability kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (quantitative)
Species reactivityReacts with: Mammals, Other species
Fixable Cell Viability Assay Kit (Fluorometric - Orange) | Cytopainter (ab176743) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/ Em = 547/573 nm. The Excitation source is 488nm or 561 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests DMSO 1 x 200µl Tracking dye Orange 1 vial
RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
- cell tracking
ab176743 has not yet been referenced specifically in any publications.