Overview

  • Product name

    Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter
    See all Cell Viability kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) | Cytopainter (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.


    This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.


    The dye is designed to label cells at Ex/Em = 398/550 nm excited with a violet laser at 405 nm.

  • Notes

    Related assays

    Review the cell health assay guide to learn about kits to perform a cell viability assaycytotoxicity assay and cell proliferation assay

  • Platform

    Flow cytometer

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 tests
    DMSO 1 x 200µl
    Tracking dye Violet 550 1 vial
  • Research areas

  • Relevance

    Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
  • Alternative names

    • cell tracking

Images

  • HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) (ab176740) in a Costar black wall/clear bottom 96-well plate.

  • Spectrum for ab176740.

Protocols

References

ab176740 has not yet been referenced specifically in any publications.

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