Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Product nameFixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter
See all Cell Viability kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (quantitative)
Species reactivityReacts with: Mammals, Other species
Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) | Cytopainter (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.
This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.
The dye is designed to label cells at Ex/Em = 398/550 nm excited with a violet laser at 405 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests DMSO 1 x 200µl Tracking dye Violet 550 1 vial
RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
- cell tracking
ab176740 has not yet been referenced specifically in any publications.