- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-FJX1 antibody
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DescriptionRabbit polyclonal to FJX1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide derived from the N terminal of human FJX1
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferConstituent: Whole serum
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab80264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 23922772 | |
| WB | 1/500 - 1/5000. Predicted molecular weight: 49 kDa. |
Target
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RelevanceFJX1 is the human ortholog of mouse and Drosophila four-jointed gene product. The Drosophila protein is important for growth and differentiation of legs and wings, and for proper development of the eyes. The exact function of this gene in humans is not known.
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Cellular localizationSecreted
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Database links
- Entrez Gene: 24147 Human
- Omim: 612206 Human
- SwissProt: Q86VR8 Human
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Alternative names
- FJX1 four jointed box 1 Drosophila antibody
- FLJ22416 antibody
- FLJ25593 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FJX1 antibody (ab80264)Image from Al-Greene NT et al., PLoS One. 2013;8(7):e69660. Fig 3.; doi: 10.1371/journal.pone.0069660.ab80264 staining FJX1 by ICC/IF (Immunocytochemistry/immunofluorescence) in HEK293T cells stably expressing MYC-tagged FJX1 dual stained for FJX1 (i, green) and MYC (ii, red) or FJX1 (iv, green) and the Golgi marker, GM130 (v, red). Nuclei were stained with 4',6-diamidino-2-phenylindole (blue). Respective merged images are shown (iii, vi). Scale bar = 100 μm
Cells were fixed with 4% paraformaldehyde, blocked with 3% BSA and incubated with primary antibody and an Alexa Fluor® was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Al-Greene NT et al. Four jointed box 1 promotes angiogenesis and is associated with poor patient survival in colorectal carcinoma. PLoS One 8:e69660 (2013). ICC/IF ; Human . Read more (PubMed: 23922772) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"