Question (71202) | FK506 (Tacrolimus), Immunosuppressant (ab120223)

Go to datasheet (ab120223)

Question

Actually the customer needs to inject mice and not human being. So all the questions below refer to in vivo experiment with the mouse:
1. Has ab120223 been used in in vivo experiments in general and for the rejection of the implant especially.

2. How should ab120223 be delivered in vivo? If it is injection, what type?

3. How should ab120223 be dissolved for in vivo experiments? For how long it will be stable in this case?

4. Is ab120223 oxidized due to the exposure to oxygen (from the air).

5. Is ab120223 delivered as a powder?

Do you have some way to help me with these?

Your assistance is appreciated.

Thanks in advance for the reply.

Best Regards,

Answer

Thank you for contacting us.
1. Has ab120223 been used in in-vivo experiments in general and for the rejection of the implant especially.
We have not tested in our laboratory but the same chemical has been published used for in-vivo studies.
http://www.ncbi.nlm.nih.gov/pubmed/22487280
http://www.sciencedirect.com/science/article/pii/S0966327406000694
http://www.ncbi.nlm.nih.gov/pubmed/22260796?dopt=Abstract
2. How should ab120223 be delivered in vivo? If it is injection, what type?
Please check the below publication and find how other people have used because we have not tested this.
3. How should ab120223 be dissolved for in vivo experiments? For how long it will be stable in this case?
The drug seems insoluble in water. We recommend dissolving in DMSO. You may check the following publication for further information;
http://sitemaker.umich.edu/amidon.papers/view_publications/da.data/1518070/Publication/gla__221_02.pdf
4. Is ab120223 oxidized due to the exposure to oxygen (from the air).
I have sent an email to lab and will let you know later.
5. Is ab120223 delivered as a powder?
It will be delivered as powder.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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