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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP12.
(Peptide available as ab25840.)
Our Abpromise guarantee covers the use of ab24373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP1A knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab24373 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab24373 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP1A knockout cells. Wild-type and FKBP1A knockout samples were subjected to SDS-PAGE. ab24373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab24373 staining FKBP12 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde before heat mediated antigen retrieval and then blocked for 45 minutes at 20°C (5 minutes of peroxidase block and 10 minutes of protein block). The primary antibody was diluted, 1/2000 in PBS and incubated with sample for 45 minutes at 21°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
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