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    fkbp12-antibody-ab2918.pdf

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Immunology Immune Interventions Transplantation
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Anti-FKBP12 antibody (ab2918)

  • Datasheet
  • SDS
Reviews (22)Q&A (2)References (26)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)

Key features and details

  • Rabbit polyclonal to FKBP12
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FKBP12 antibody
    See all FKBP12 primary antibodies
  • Description

    Rabbit polyclonal to FKBP12
  • Host species

    Rabbit
  • Specificity

    Detects FK506 binding protein 12 kDa (FKBP12).
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human FKBP12 aa 1-13 (N terminal).
    Sequence:

    GVQVETISPGDGR


    (Peptide available as ab4935)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune Interventions
    • Transplantation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant human FKBP12 protein (ab85840)
  • Recombinant Protein

    • Recombinant human FKBP12 protein (ab85840)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (3)
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P62942 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2280 Human
    • Entrez Gene: 379069 Xenopus laevis
    • Omim: 186945 Human
    • SwissProt: P18203 Cow
    • SwissProt: P62942 Human
    • SwissProt: P62943 Rabbit
    • SwissProt: O42123 Xenopus laevis
    • Unigene: 471933 Human
    • Unigene: 700839 Human
    • Unigene: 960 Xenopus laevis
    see all
  • Alternative names

    • 12 kDa FK506-binding protein antibody
    • 12 kDa FKBP antibody
    • Calstabin 1 antibody
    • FK506 binding protein 1 antibody
    • FK506 binding protein 12 antibody
    • FK506 binding protein 1A (12kD) antibody
    • FK506 binding protein 1A 12kDa antibody
    • FK506 binding protein 1A antibody
    • FK506 binding protein T cell 12 kD antibody
    • FK506 binding protein, T cell, 12 kD antibody
    • FK506 binding protein12 antibody
    • FK506-binding protein 1A antibody
    • FKB1A_HUMAN antibody
    • FKBP 12 antibody
    • FKBP 1A antibody
    • FKBP-12 antibody
    • FKBP-1A antibody
    • FKBP1 antibody
    • FKBP12 antibody
    • FKBP12 Exip3 antibody
    • FKBP12C antibody
    • fkbp1a antibody
    • Immunophilin FKBP12 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
    • PKC12 antibody
    • PKCI2 antibody
    • PPIase antibody
    • PPIase FKBP1A antibody
    • Protein kinase C inhibitor 2 antibody
    • Rotamase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/50 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
    ICC/IF image of ab2918 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2918, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/20 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)This image is courtesy of an Abreview submitted by Miss gwen lagoda

    ab2918 at 1/1000 staining FKBP12 from human penis tissue by IHC-P. The tissue was paraformaldehyde fixed and the slides were incubated in citrate buffer for antigen retrieval and heated for 20 minutes. The tissue was incubated with ab2918 for 24 hours. The secondary antibody used was part of the DAKO ENVISON System. The picture depicts staining of nerve fibers within corporal tissue of the human penis.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)This image is courtesy of an Abreview submitted by Miss gwen lagoda
    ab2918 at 1/1000 staining mouse heart tissue sections by IHC-P. The tissue was paraformaldehyde fixed, blocked and then a citrate buffer / heat mediated antigen retireival step was performed. The tissue was incubated with the antibody for 24 hours. An HRP conjugated rabbit polyclonal antibody was used as the secondary.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (26)

Publishing research using ab2918? Please let us know so that we can cite the reference in this datasheet.

ab2918 has been referenced in 26 publications.

  • Wyckelsma VL  et al. Loss of a-actinin-3 during human evolution provides superior cold resilience and muscle heat generation. Am J Hum Genet 108:446-457 (2021). PubMed: 33600773
  • Ishizaki T  et al. cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1 (AMA1) in Plasmodium yoelii. Parasitol Int 85:102435 (2021). PubMed: 34390881
  • Saadeh K  et al. Protein expression profiles in murine ventricles modeling catecholaminergic polymorphic ventricular tachycardia: effects of genotype and sex. Ann N Y Acad Sci 1478:63-74 (2020). PubMed: 32713021
  • Buckley C  et al. FK506 regulates Ca2+ release evoked by inositol 1,4,5-trisphosphate independently of FK-binding protein in endothelial cells. Br J Pharmacol 177:1131-1149 (2020). PubMed: 31705533
  • Asghari P  et al. Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification. Elife 9:N/A (2020). PubMed: 31916935
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FKBP12 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Gwen Lagoda

Verified customer

Submitted Apr 26 2007

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FKBP12 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Penis Sections)
Specification
Penis Sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Gwen Lagoda

Verified customer

Submitted Apr 12 2007

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FKBP12 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Penis Tissue)
Specification
Penis Tissue
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Gwen Lagoda

Verified customer

Submitted Dec 07 2006

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