Product nameAnti-FKBP12 antibody [EP3887]
See all FKBP12 primary antibodies
DescriptionRabbit monoclonal [EP3887] to FKBP12
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human FKBP12 aa 1-100 (internal sequence). The exact sequence is proprietary.
- HL-60, U937 and SH-SY5Y lysates; HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 12 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionMay play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similaritiesBelongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain.
- Information by UniProt
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- Calstabin 1 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108420 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108420 was shown to recognize FKBP12 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP12 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab108420 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-FKBP12 antibody [EP3887] (ab108420) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HL-60 brain lysate
Lane 3 : U937 lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 12 kDa
Immunofluorescent staining of FKBP12 in HeLa cells using ab108420 at 1/50 dilution.
ab108420 has not yet been referenced specifically in any publications.