- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-FKBP12 antibody [EPR3888]
See all FKBP12 primary antibodies -
DescriptionRabbit monoclonal [EPR3888] to FKBP12
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human FKBP12 aa 50-150 (C terminal). The exact sequence is proprietary.
-
Positive control
- Fetal brain, U937 and SH-SY5Y cell lysates
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR3888
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab92459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ICC |
WB: 1/10,000 - 1/50,000. Predicted molecular weight: 12 kDa.
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
FunctionMay play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
-
Sequence similaritiesBelongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain. -
Cellular localizationCytoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 2280 Human
- Entrez Gene: 14225 Mouse
- Entrez Gene: 25639 Rat
- Omim: 186945 Human
- SwissProt: P62942 Human
- SwissProt: P26883 Mouse
- SwissProt: Q62658 Rat
- Unigene: 471933 Human
see all -
Alternative names
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- Calstabin 1 antibody
see all
Images
-
![Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)](https://www.abcam.com/ps/products/92/ab92459/Images/ab92459-297787-ab92459KOWBGDB.jpg)
Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab92459 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92459 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP12 knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab92459 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)](https://www.abcam.com/ps/products/92/ab92459/Images/FKBP12-Primary-antibodies-ab92459-1.JPG)
Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : U937 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 12 kDa
References
ab92459 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"