Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-FKBP12 antibody [EPR3888] (ab92459)

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Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
  • Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
  • Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
  • Anti-FKBP12 antibody [EPR3888] (ab92459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3888] to FKBP12
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-FKBP12 antibody [EPR3888]
    See all FKBP12 primary antibodies

  • Description

    Rabbit monoclonal [EPR3888] to FKBP12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human FKBP12 aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control

    • U937 and SH-SY5Y cell lysates. Fetal brain, human brain lysate, mouse heart lysate and rat heart tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3888

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/2000. Predicted molecular weight: 12 kDa.

For unpurified use at 1/10000 - 1/50000
Notes
WB
1/2000. Predicted molecular weight: 12 kDa.

For unpurified use at 1/10000 - 1/50000
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Function

    May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

  • Sequence similarities

    Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
    Contains 1 PPIase FKBP-type domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 12 kDa FK506-binding protein antibody
    • 12 kDa FKBP antibody
    • Calstabin 1 antibody
    • FK506 binding protein 1 antibody
    • FK506 binding protein 12 antibody
    • FK506 binding protein 1A (12kD) antibody
    • FK506 binding protein 1A 12kDa antibody
    • FK506 binding protein 1A antibody
    • FK506 binding protein T cell 12 kD antibody
    • FK506 binding protein, T cell, 12 kD antibody
    • FK506 binding protein12 antibody
    • FK506-binding protein 1A antibody
    • FKB1A_HUMAN antibody
    • FKBP 12 antibody
    • FKBP 1A antibody
    • FKBP-12 antibody
    • FKBP-1A antibody
    • FKBP1 antibody
    • FKBP12 antibody
    • FKBP12 Exip3 antibody
    • FKBP12C antibody
    • fkbp1a antibody
    • Immunophilin FKBP12 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
    • PKC12 antibody
    • PKCI2 antibody
    • PPIase antibody
    • PPIase FKBP1A antibody
    • Protein kinase C inhibitor 2 antibody
    • Rotamase antibody
    see all

Images

  • Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
    Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)
    Lane 4: Human brain whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92459 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab92459 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP12 knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab92459 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
    Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
    All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/2000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
    Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
    All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : U937 cell lysate
    Lane 3 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 12 kDa

  • Anti-FKBP12 antibody [EPR3888] (ab92459)
    Anti-FKBP12 antibody [EPR3888] (ab92459)

References (0)

Publishing research using ab92459? Please let us know so that we can cite the reference in this datasheet.

ab92459 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-FKBP12 antibody [EPR3888]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (cultured cell)
Gel Running Conditions
Reduced Denaturing (4%-12% bis tris)
Loading amount
30 µg
Specification
cultured cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 09 2016

Western blot abreview for Anti-FKBP12 antibody [EPR3888]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Exponential growth)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
10 µg
Specification
Exponential growth
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Nov 09 2015

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