Recombinant Anti-FKBP12 antibody [EPR3888] (ab92459)
![Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)](https://www.abcam.com/ps/products/92/ab92459/Images/ab92459-297787-anti-fkbp12-antibody-epr3888-western-blot.jpg "Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3888] to FKBP12
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FKBP12 antibody [EPR3888]
See all FKBP12 primary antibodies -
Description
Rabbit monoclonal [EPR3888] to FKBP12 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human FKBP12 aa 50-150 (C terminal). The exact sequence is proprietary.
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Positive control
- U937 and SH-SY5Y cell lysates. Fetal brain, human brain lysate, mouse heart lysate and rat heart tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3888 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/2000. Predicted molecular weight: 12 kDa.
For unpurified use at 1/10000 - 1/50000 |
| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 12 kDa. For unpurified use at 1/10000 - 1/50000 |
Target
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Function
May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Contains 1 PPIase FKBP-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2280 Human
- Entrez Gene: 14225 Mouse
- Entrez Gene: 25639 Rat
- Omim: 186945 Human
- SwissProt: P62942 Human
- SwissProt: P26883 Mouse
- SwissProt: Q62658 Rat
- Unigene: 471933 Human
see all -
Alternative names
- 12 kDa FK506-binding protein antibody
- 12 kDa FKBP antibody
- Calstabin 1 antibody
see all
Images
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Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab92459 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92459 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP12 knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab92459 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)](https://www.abcam.com/ps/products/92/ab92459/Images/ab92459-333659-anti-fkbp12-antibody-epr3888-western-blot.jpg)
Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/2000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDaBlocking Buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)](https://www.abcam.com/ps/products/92/ab92459/Images/ab92459-107462-anti-fkbp12-antibody-epr3888-western-blot.jpg)
Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : U937 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 12 kDa -
![Anti-FKBP12 antibody [EPR3888] (ab92459)](https://www.abcam.com/ps/products/92/ab92459/Images/ab92459-4-benefits-of-recombinant-antibodies.png)
Anti-FKBP12 antibody [EPR3888] (ab92459)
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab92459 has not yet been referenced specifically in any publications.
