Validated using a knockout cell line
Recombinant
RabMAb

Anti-FKBP12 antibody [EPR3888] (ab92459)

Overview

  • Product name
    Anti-FKBP12 antibody [EPR3888]
    See all FKBP12 primary antibodies
  • Description
    Rabbit monoclonal [EPR3888] to FKBP12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FKBP12 aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Fetal brain, U937 and SH-SY5Y cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3888
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab92459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notes
    ICC: 1/50 - 1/100.
    WB: 1/10,000 - 1/50,000. Predicted molecular weight: 12 kDa.

    Is unsuitable for Flow Cyt, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
    • Sequence similarities
      Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
      Contains 1 PPIase FKBP-type domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12 kDa FK506-binding protein antibody
      • 12 kDa FKBP antibody
      • Calstabin 1 antibody
      • FK506 binding protein 1 antibody
      • FK506 binding protein 12 antibody
      • FK506 binding protein 1A (12kD) antibody
      • FK506 binding protein 1A 12kDa antibody
      • FK506 binding protein 1A antibody
      • FK506 binding protein T cell 12 kD antibody
      • FK506 binding protein, T cell, 12 kD antibody
      • FK506 binding protein12 antibody
      • FK506-binding protein 1A antibody
      • FKB1A_HUMAN antibody
      • FKBP 12 antibody
      • FKBP 1A antibody
      • FKBP-12 antibody
      • FKBP-1A antibody
      • FKBP1 antibody
      • FKBP12 antibody
      • FKBP12 Exip3 antibody
      • FKBP12C antibody
      • fkbp1a antibody
      • Immunophilin FKBP12 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
      • PKC12 antibody
      • PKCI2 antibody
      • PPIase antibody
      • PPIase FKBP1A antibody
      • Protein kinase C inhibitor 2 antibody
      • Rotamase antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)
      Lane 4: Human brain whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab92459 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab92459 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP12 knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab92459 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : U937 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 12 kDa

    References

    ab92459 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (cultured cell)
    Gel Running Conditions
    Reduced Denaturing (4%-12% bis tris)
    Loading amount
    30 µg
    Specification
    cultured cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 09 2016

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Exponential growth)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    10 µg
    Specification
    Exponential growth
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 09 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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