Application
Western blot
Sample
Mouse Cell lysate - whole cell (cultured cell)
Gel Running Conditions
Reduced Denaturing (4%-12% bis tris)
Loading amount
30 µg
Specification
cultured cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% milk in TBST
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-Rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 12 kDa
Abcam user community
Verified customer
Submitted Mar 09 2016