Key features and details
- Rabbit polyclonal to FKBP135
- Suitable for: IP
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-FKBP135 antibody
See all FKBP135 primary antibodies
DescriptionRabbit polyclonal to FKBP135
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Chimpanzee
Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa960 and aa1020 of human FKBP135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).
General notesCentrifuge tube to remove product from lid.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was purified using an epitope specific to FKBP135 immobilized on solid support.
Our Abpromise guarantee covers the use of ab14433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 1 to 4 mcg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 148 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Prepare working dilution immediately before use.
FunctionMay be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.
Sequence similaritiesBelongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain.
DomainThe PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
The central coiled-coil region is responsible for association with early endosomes.
Cellular localizationCytoplasm. Cell projection > axon. Early endosome. Present in axons and neuronal growth cones.
- Information by UniProt
- 133 kDa FK506-binding protein antibody
- 133 kDa FKBP antibody
- C430014M02Rik antibody
Detection of Human FKBP-135 by Western Blot and Immunoprecipitation. Samples: Nuclear extract (5 and 20 mcg for WB; 1 mg for IP) from HeLa cells. Antibodies: ab14432 used at 0.2 mcg/ml for WB in lane 1,2,3,4,5 and at 2 µg/mg extract for IP in lane 3 (B), ab14433 used at 2 µg/mg extract for IP in lane 4 (B) and ab14434 used at 2 mcg/mg extract for IP in lane 5 (B). Detection: chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).
ab14433 has not yet been referenced specifically in any publications.