Anti-FKBP25 antibody (ab16654)
Key features and details
- Rabbit polyclonal to FKBP25
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FKBP25 antibody
See all FKBP25 primary antibodies -
Description
Rabbit polyclonal to FKBP25 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP25.
Read Abcam's proprietary immunogen policy (Peptide available as ab16979.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
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| All applications |
Rabbit
Hamster
Cow
Monkey
African green monkey
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
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| Notes |
|---|
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WB
Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins. -
Sequence similarities
Belongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515069 Cow
- Entrez Gene: 2287 Human
- Entrez Gene: 30795 Mouse
- Entrez Gene: 100009004 Rabbit
- Entrez Gene: 299104 Rat
- Omim: 186947 Human
- SwissProt: P26884 Cow
- SwissProt: Q00688 Human
see all -
Alternative names
- 25 kDa FK506-binding protein antibody
- 25 kDa FKBP antibody
- FK506 binding protein 25 T cell antibody
see all
Images
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Western blot - Anti-FKBP25 antibody (ab16654)All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Lysate
Lane 3 : HeLa Whole Cell Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lane 4 : HeLa Nuclear Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Western blot - Anti-FKBP25 antibody (ab16654)This image is courtesy of collaborator dataAll lanes : Anti-FKBP25 antibody (ab16654) at 0.05 µg/ml
Lane 1 : HeLa nuclear extracts
Lane 2 : HeLa nuclear extracts with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lane 3 : HeLa nuclear extracts with Human FKBP25 peptide (ab16980) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDa
ab16654 specifically recognises FKBP25 (lane1), which can be blocked using the N-terminal immunizing peptide (lane2) but not the corresponding C-terminal peptide (lane3). -
Western blot - Anti-FKBP25 antibody (ab16654)All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab16654 has been referenced in 5 publications.
- Tong J et al. FKBP3 mediates oxaliplatin resistance in colorectal cancer cells by regulating HDAC2 expression. Oncol Rep N/A:N/A (2019). PubMed: 31524278
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Zhu W et al. FKBP3 Promotes Proliferation of Non-Small Cell Lung Cancer Cells through Regulating Sp1/HDAC2/p27. Theranostics 7:3078-3089 (2017). PubMed: 28839465
- Schreiber KH et al. Rapamycin-mediated mTORC2 inhibition is determined by the relative expression of FK506-binding proteins. Aging Cell 14:265-73 (2015). WB . PubMed: 25652038
- Ochocka AM et al. FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53. FEBS Lett 583:621-6 (2009). WB ; Human . PubMed: 19166840