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    fkbp25-antibody-ab16654.pdf

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Epigenetics and Nuclear Signaling Transcription Co-factors
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Anti-FKBP25 antibody (ab16654)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (5)

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Western blot - Anti-FKBP25 antibody (ab16654)
  • Western blot - Anti-FKBP25 antibody (ab16654)
  • Western blot - Anti-FKBP25 antibody (ab16654)

Key features and details

  • Rabbit polyclonal to FKBP25
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human FKBP25 protein (ab93683)

View more associated products

Overview

  • Product name

    Anti-FKBP25 antibody
    See all FKBP25 primary antibodies
  • Description

    Rabbit polyclonal to FKBP25
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP25.

    Read Abcam's proprietary immunogen policy (Peptide available as ab16979.)
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Recombinant human FKBP25 protein (ab93683)
  • Recombinant Protein

    • Recombinant human FKBP25 protein (ab93683)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Rabbit
Hamster
Cow
Monkey
African green monkey
Application Abreviews Notes
WB (5)
Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
Notes
WB
Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
  • Sequence similarities

    Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q00688 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515069 Cow
    • Entrez Gene: 2287 Human
    • Entrez Gene: 30795 Mouse
    • Entrez Gene: 100009004 Rabbit
    • Entrez Gene: 299104 Rat
    • Omim: 186947 Human
    • SwissProt: P26884 Cow
    • SwissProt: Q00688 Human
    • SwissProt: Q62446 Mouse
    • SwissProt: O46638 Rabbit
    • Unigene: 509226 Human
    • Unigene: 28480 Mouse
    • Unigene: 1464 Rat
    see all
  • Alternative names

    • 25 kDa FK506-binding protein antibody
    • 25 kDa FKBP antibody
    • FK506 binding protein 25 T cell antibody
    • FK506 binding protein 25, T cell, 25-KD antibody
    • FK506 binding protein 3 (25kD) antibody
    • FK506-binding protein 3 antibody
    • FKBP 25 antibody
    • FKBP 3 antibody
    • FKBP-25 antibody
    • FKBP-3 antibody
    • FKBP25 antibody
    • FKBP3 antibody
    • FKBP3_HUMAN antibody
    • Immunophilin FKBP25 antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP3 antibody
    • PPIase antibody
    • PPIase FKBP3 antibody
    • Rapamycin binding protein antibody
    • Rapamycin-selective 25 kDa immunophilin antibody
    • Rotamase antibody
    see all

Images

  • Western blot - Anti-FKBP25 antibody (ab16654)
    Western blot - Anti-FKBP25 antibody (ab16654)
    All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : HeLa Nuclear Lysate
    Lane 3 : HeLa Whole Cell Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
    Lane 4 : HeLa Nuclear Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Predicted band size: 25 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-FKBP25 antibody (ab16654)
    Western blot - Anti-FKBP25 antibody (ab16654)This image is courtesy of collaborator data
    All lanes : Anti-FKBP25 antibody (ab16654) at 0.05 µg/ml

    Lane 1 : HeLa nuclear extracts
    Lane 2 : HeLa nuclear extracts with Human FKBP25 peptide (ab16979) at 1 µg/ml
    Lane 3 : HeLa nuclear extracts with Human FKBP25 peptide (ab16980) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 25 kDa



    ab16654 specifically recognises FKBP25 (lane1), which can be blocked using the N-terminal immunizing peptide (lane2) but not the corresponding C-terminal peptide (lane3).
  • Western blot - Anti-FKBP25 antibody (ab16654)
    Western blot - Anti-FKBP25 antibody (ab16654)
    All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?
    Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab16654? Please let us know so that we can cite the reference in this datasheet.

ab16654 has been referenced in 5 publications.

  • Tong J  et al. FKBP3 mediates oxaliplatin resistance in colorectal cancer cells by regulating HDAC2 expression. Oncol Rep N/A:N/A (2019). PubMed: 31524278
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
  • Zhu W  et al. FKBP3 Promotes Proliferation of Non-Small Cell Lung Cancer Cells through Regulating Sp1/HDAC2/p27. Theranostics 7:3078-3089 (2017). PubMed: 28839465
  • Schreiber KH  et al. Rapamycin-mediated mTORC2 inhibition is determined by the relative expression of FK506-binding proteins. Aging Cell 14:265-73 (2015). WB . PubMed: 25652038
  • Ochocka AM  et al. FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53. FEBS Lett 583:621-6 (2009). WB ; Human . PubMed: 19166840

Customer reviews and Q&As

Show All Reviews Q&A
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1-7 of 7 Abreviews or Q&A

Immunoprecipitation abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
500 µg
Specification
293T
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

Submitted Oct 30 2007

Immunoprecipitation abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12)
Total protein in input
500 µg
Specification
PC12
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

Submitted Oct 30 2007

Western blot abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
No blocking step used for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Sep 20 2007

Western blot abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Sep 20 2007

Western blot abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Sep 20 2007

Western blot abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Jun 20 2007

Western blot abreview for Anti-FKBP25 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
20 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Jun 18 2007

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