Anti-FKBP25 antibody (ab16654)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-FKBP25 antibody
See all FKBP25 primary antibodies -
DescriptionRabbit polyclonal to FKBP25
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Hamster, Human, African green monkey
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP25.
Read Abcam's proprietary immunogen policy (Peptide available as ab16979.) -
Positive control
- HeLa nuclear extracts HeLa Whole Cell Lysate HeLa Nuclear Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/1000. | |
| WB | Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionFK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
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Sequence similaritiesBelongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 515069 Cow
- Entrez Gene: 2287 Human
- Entrez Gene: 30795 Mouse
- Entrez Gene: 100009004 Rabbit
- Entrez Gene: 299104 Rat
- Omim: 186947 Human
- SwissProt: P26884 Cow
- SwissProt: Q00688 Human
see all -
Alternative names
- 25 kDa FK506-binding protein antibody
- 25 kDa FKBP antibody
- FK506 binding protein 25 T cell antibody
see all
Images
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Western blot - Anti-FKBP25 antibody (ab16654)All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Lysate
Lane 3 : HeLa Whole Cell Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lane 4 : HeLa Nuclear Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Western blot - Anti-FKBP25 antibody (ab16654)This image is courtesy of collaborator dataAll lanes : Anti-FKBP25 antibody (ab16654) at 0.05 µg/ml
Lane 1 : HeLa nuclear extracts
Lane 2 : HeLa nuclear extracts with Human FKBP25 peptide (ab16979) at 1 µg/ml
Lane 3 : HeLa nuclear extracts with Human FKBP25 peptide (ab16980) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDa
ab16654 specifically recognises FKBP25 (lane1), which can be blocked using the N-terminal immunizing peptide (lane2) but not the corresponding C-terminal peptide (lane3). -
Immunoprecipitation - Anti-FKBP25 antibody (ab16654)This image is courtesy of an anonymous Abreviewab16654 immunoprecipitating FKBP25 from rat PC12 whole cell lysate. A protein A matrix was used and the antibody was used at 4ug/mg of the lysate. Ab16654 was used in subsequent western blot (Lane 1). Lane 2 shows control IgG immunoprecipitate, Lane 3 shows input material.
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Western blot - Anti-FKBP25 antibody (ab16654)All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP25 antibody (ab16654)Image courtesy of Human Protein Atlas
ab16654 staining FKBP25 in human testis, showing a distinct and strong staining pattern at cells in ductus seminiferus. Paraffin embedded human skin tissue was incubated with ab16654 (1/1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
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ab16654 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Datasheets and documents
References
This product has been referenced in:
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »
- Zhu W et al. FKBP3 Promotes Proliferation of Non-Small Cell Lung Cancer Cells through Regulating Sp1/HDAC2/p27. Theranostics 7:3078-3089 (2017). Read more (PubMed: 28839465) »
