Anti-FKBP25 antibody (ab168772)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-FKBP25 antibody
See all FKBP25 primary antibodies -
DescriptionMouse polyclonal to FKBP25
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
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Immunogen
Full length protein, corresponding to amino acids 1-224 of Human FKBP25 (NP_002004.1, UniProt ID: Q00688).
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Positive control
- FKBP25-transfected 293T cell lysate; Human liver tissue lysate; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab168772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 25 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. For IF applications antibody purification with Protein A will be needed prior to use. |
Target
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FunctionFK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
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Sequence similaritiesBelongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 515069 Cow
- Entrez Gene: 2287 Human
- Entrez Gene: 30795 Mouse
- Entrez Gene: 100009004 Rabbit
- Omim: 186947 Human
- SwissProt: P26884 Cow
- SwissProt: Q00688 Human
- SwissProt: Q62446 Mouse
see all -
Alternative names
- 25 kDa FK506-binding protein antibody
- 25 kDa FKBP antibody
- FK506 binding protein 25 T cell antibody
see all
Images
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Western blot - Anti-FKBP25 antibody (ab168772)All lanes : Anti-FKBP25 antibody (ab168772) at 1/500 dilution
Lane 1 : FKBP25-transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa -
Western blot - Anti-FKBP25 antibody (ab168772)Anti-FKBP25 antibody (ab168772) at 1/500 dilution + Human liver tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 25 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FKBP25 using purified ab168772 at 10 µg/ml. Antibody purification with Protein A will be needed prior to use.
Datasheets and documents
References
ab168772 has not yet been referenced specifically in any publications.
