Anti-FKBP25 antibody (ab168772)
Key features and details
- Mouse polyclonal to FKBP25
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FKBP25 antibody
See all FKBP25 primary antibodies -
Description
Mouse polyclonal to FKBP25 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length protein, corresponding to amino acids 1-224 of Human FKBP25 (NP_002004.1, UniProt ID: Q00688).
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Positive control
- FKBP25-transfected 293T cell lysate; Human liver tissue lysate; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rabbit
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml. For IF applications antibody purification with Protein A will be needed prior to use.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. For IF applications antibody purification with Protein A will be needed prior to use. |
Target
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Function
FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins. -
Sequence similarities
Belongs to the FKBP-type PPIase family.
Contains 1 PPIase FKBP-type domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515069 Cow
- Entrez Gene: 2287 Human
- Entrez Gene: 30795 Mouse
- Entrez Gene: 100009004 Rabbit
- Omim: 186947 Human
- SwissProt: P26884 Cow
- SwissProt: Q00688 Human
- SwissProt: Q62446 Mouse
see all -
Alternative names
- 25 kDa FK506-binding protein antibody
- 25 kDa FKBP antibody
- FK506 binding protein 25 T cell antibody
see all
Images
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Western blot - Anti-FKBP25 antibody (ab168772)All lanes : Anti-FKBP25 antibody (ab168772) at 1/500 dilution
Lane 1 : FKBP25-transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa -
Western blot - Anti-FKBP25 antibody (ab168772)Anti-FKBP25 antibody (ab168772) at 1/500 dilution + Human liver tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 25 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FKBP25 using purified ab168772 at 10 µg/ml. Antibody purification with Protein A will be needed prior to use.
Protocols
Datasheets and documents
References (0)
ab168772 has not yet been referenced specifically in any publications.
