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    fkbp25-antibody-ab168772.pdf

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Epigenetics and Nuclear Signaling Transcription Co-factors
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Anti-FKBP25 antibody (ab168772)

  • Datasheet
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Western blot - Anti-FKBP25 antibody (ab168772)
  • Western blot - Anti-FKBP25 antibody (ab168772)
  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)

Key features and details

  • Mouse polyclonal to FKBP25
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
Product image
Recombinant human FKBP25 protein (ab93683)

View more associated products

Overview

  • Product name

    Anti-FKBP25 antibody
    See all FKBP25 primary antibodies
  • Description

    Mouse polyclonal to FKBP25
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length protein, corresponding to amino acids 1-224 of Human FKBP25 (NP_002004.1, UniProt ID: Q00688).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • FKBP25-transfected 293T cell lysate; Human liver tissue lysate; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Positive Controls

    • Recombinant human FKBP25 protein (ab93683)
  • Recombinant Protein

    • Recombinant human FKBP25 protein (ab93683)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rabbit
Cow
Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
ICC/IF
Use a concentration of 10 µg/ml. For IF applications antibody purification with Protein A will be needed prior to use.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
ICC/IF
Use a concentration of 10 µg/ml. For IF applications antibody purification with Protein A will be needed prior to use.

Target

  • Function

    FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
  • Sequence similarities

    Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q00688 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515069 Cow
    • Entrez Gene: 2287 Human
    • Entrez Gene: 30795 Mouse
    • Entrez Gene: 100009004 Rabbit
    • Omim: 186947 Human
    • SwissProt: P26884 Cow
    • SwissProt: Q00688 Human
    • SwissProt: Q62446 Mouse
    • SwissProt: O46638 Rabbit
    • Unigene: 509226 Human
    • Unigene: 28480 Mouse
    see all
  • Alternative names

    • 25 kDa FK506-binding protein antibody
    • 25 kDa FKBP antibody
    • FK506 binding protein 25 T cell antibody
    • FK506 binding protein 25, T cell, 25-KD antibody
    • FK506 binding protein 3 (25kD) antibody
    • FK506-binding protein 3 antibody
    • FKBP 25 antibody
    • FKBP 3 antibody
    • FKBP-25 antibody
    • FKBP-3 antibody
    • FKBP25 antibody
    • FKBP3 antibody
    • FKBP3_HUMAN antibody
    • Immunophilin FKBP25 antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP3 antibody
    • PPIase antibody
    • PPIase FKBP3 antibody
    • Rapamycin binding protein antibody
    • Rapamycin-selective 25 kDa immunophilin antibody
    • Rotamase antibody
    see all

Images

  • Western blot - Anti-FKBP25 antibody (ab168772)
    Western blot - Anti-FKBP25 antibody (ab168772)
    All lanes : Anti-FKBP25 antibody (ab168772) at 1/500 dilution

    Lane 1 : FKBP25-transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 25 kDa

  • Western blot - Anti-FKBP25 antibody (ab168772)
    Western blot - Anti-FKBP25 antibody (ab168772)
    Anti-FKBP25 antibody (ab168772) at 1/500 dilution + Human liver tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FKBP25 using purified ab168772 at 10 µg/ml. Antibody purification with Protein A will be needed prior to use.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab168772? Please let us know so that we can cite the reference in this datasheet.

    ab168772 has not yet been referenced specifically in any publications.

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