Overview

  • Product name
    Anti-FKBP25 antibody
    See all FKBP25 primary antibodies
  • Description
    Mouse polyclonal to FKBP25
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Full length protein, corresponding to amino acids 1-224 of Human FKBP25 (NP_002004.1, UniProt ID: Q00688).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • FKBP25-transfected 293T cell lysate; Human liver tissue lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
ICC/IF Use a concentration of 10 µg/ml. For IF applications antibody purification with Protein A will be needed prior to use.

Target

  • Function
    FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
  • Sequence similarities
    Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 25 kDa FK506-binding protein antibody
    • 25 kDa FKBP antibody
    • FK506 binding protein 25 T cell antibody
    • FK506 binding protein 25, T cell, 25-KD antibody
    • FK506 binding protein 3 (25kD) antibody
    • FK506-binding protein 3 antibody
    • FKBP 25 antibody
    • FKBP 3 antibody
    • FKBP-25 antibody
    • FKBP-3 antibody
    • FKBP25 antibody
    • FKBP3 antibody
    • FKBP3_HUMAN antibody
    • Immunophilin FKBP25 antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP3 antibody
    • PPIase antibody
    • PPIase FKBP3 antibody
    • Rapamycin binding protein antibody
    • Rapamycin-selective 25 kDa immunophilin antibody
    • Rotamase antibody
    see all

Images

  • Western blot - Anti-FKBP25 antibody (ab168772)
    Western blot - Anti-FKBP25 antibody (ab168772)
    All lanes : Anti-FKBP25 antibody (ab168772) at 1/500 dilution

    Lane 1 : FKBP25-transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 25 kDa

  • Western blot - Anti-FKBP25 antibody (ab168772)
    Western blot - Anti-FKBP25 antibody (ab168772)
    Anti-FKBP25 antibody (ab168772) at 1/500 dilution + Human liver tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP25 antibody (ab168772)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FKBP25 using purified ab168772 at 10 µg/ml. Antibody purification with Protein A will be needed prior to use.

References

ab168772 has not yet been referenced specifically in any publications.

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