Overview

  • Product name

    Anti-FKBP38 antibody [EPR7441(2)]
    See all FKBP38 primary antibodies
  • Description

    Rabbit monoclonal [EPR7441(2)] to FKBP38
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FKBP38 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa cells; 293T, HeLa and Jurkat whole cell lysate (ab7899).
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis.
    • Tissue specificity

      Widely expressed. Highest levels seen in the brain.
    • Sequence similarities

      Contains 1 PPIase FKBP-type domain.
      Contains 3 TPR repeats.
    • Cellular localization

      Mitochondrion membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 38 kDa FK506-binding protein antibody
      • 38 kDa FKBP antibody
      • 38kDa antibody
      • FK506 binding protein 8 antibody
      • FK506 binding protein 8, 38kDa antibody
      • FK506 binding protein, 38kDa antibody
      • FK506-binding protein 8 antibody
      • FKBP 38 antibody
      • FKBP-38 antibody
      • FKBP-8 antibody
      • Fkbp8 antibody
      • FKBP8_HUMAN antibody
      • FKBPR38 antibody
      • hFKBP38 antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP8 antibody
      • PPIase FKBP8 antibody
      • Rotamase antibody
      • Sam11 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: ProteinX knockout HAP1 cell lysate (20 µg)
      Lane 3: CellX1 cell lysate (20 µg)
      Lane 4: CellX2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab129113 observed at 51 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab129113 was shown to specifically react with FKBP38 when FKBP38 knockout samples were used. Wild-type and FKBP38 knockout samples were subjected to SDS-PAGE. ab129113 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-FKBP38 antibody [EPR7441(2)] (ab129113) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa

    • ab129113, at 1/100 dilution, staining FKBP38 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Birgisdottir ÅB  et al. Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs. Autophagy 15:1333-1355 (2019). Read more (PubMed: 30767700) »
    • Bhujabal Z  et al. FKBP8 recruits LC3A to mediate Parkin-independent mitophagy. EMBO Rep 18:947-961 (2017). Read more (PubMed: 28381481) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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