Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

Submit a review Submit a question
Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
  • Immunocytochemistry - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
  • Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
  • Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7441(2)] to FKBP38 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free
    See all FKBP38 primary antibodies

  • Description

    Rabbit monoclonal [EPR7441(2)] to FKBP38 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248311 is the carrier-free version of ab129113 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248311 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 45 kDa).
Notes
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis.

    • Tissue specificity

      Widely expressed. Highest levels seen in the brain.

    • Sequence similarities

      Contains 1 PPIase FKBP-type domain.
      Contains 3 TPR repeats.

    • Cellular localization

      Mitochondrion membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 38 kDa FK506-binding protein antibody
        • 38 kDa FKBP antibody
        • 38kDa antibody
        • FK506 binding protein 8 antibody
        • FK506 binding protein 8, 38kDa antibody
        • FK506 binding protein, 38kDa antibody
        • FK506-binding protein 8 antibody
        • FKBP 38 antibody
        • FKBP-38 antibody
        • FKBP-8 antibody
        • Fkbp8 antibody
        • FKBP8_HUMAN antibody
        • FKBPR38 antibody
        • hFKBP38 antibody
        • Peptidyl-prolyl cis-trans isomerase FKBP8 antibody
        • PPIase FKBP8 antibody
        • Rotamase antibody
        • Sam11 antibody
        see all

      Images

      • Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
        Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

        This data was developed using ab129113, the same antibody clone in a different buffer formulation.

        Lane 1: Wild-type HAP1 cell lysate (20 µg)

        Lane 2: FKBP38 knockout HAP1 cell lysate (20 µg) 

        Lane 3: HeLa cell lysate (20 µg)

        Lanes 1 - 3: Merged signal (red and green). Green - ab129113 observed at 51 kDa. Red - loading control, ab18058, observed at 124 kDa.

        ab129113 was shown to specifically react with FKBP38 when FKBP38 knockout samples were used. Wild-type and FKBP38 knockout samples were subjected to SDS-PAGE. ab129113 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      • Immunocytochemistry - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
        Immunocytochemistry - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
        This data was developed using ab129113, the same antibody clone in a different buffer formulation.ab129113, at 1/100 dilution, staining FKBP38 in HeLa cells by Immunofluorescence.
      • Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
        Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
        All lanes : Anti-FKBP38 antibody [EPR7441(2)] (ab129113) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 45 kDa



        This data was developed using ab129113, the same antibody clone in a different buffer formulation.

      • Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
        Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248311? Please let us know so that we can cite the reference in this datasheet.

      ab248311 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab248311.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com