Product nameAnti-FKBP51 antibody
See all FKBP51 primary antibodies
DescriptionRabbit polyclonal to FKBP51
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human FKBP51 aa 407-457. The exact sequence is proprietary.
Database link: Q13451
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInteracts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
Tissue specificityWidely expressed, enriched in testis compared to other tissues.
Sequence similaritiesContains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for FKBP51 using ab264207 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB.
All lanes : Anti-FKBP51 antibody (ab264207) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 seconds
FKBP51 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab264207 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264207 at 1 µg/ml.
Lane 1: ab264207 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264207 has not yet been referenced specifically in any publications.