Anti-FKBP51 antibody (ab2901)
Key features and details
- Rabbit polyclonal to FKBP51
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-FKBP51 antibody
See all FKBP51 primary antibodies -
Description
Rabbit polyclonal to FKBP51 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- WB: K562, HepG2, PC3, HeLa and MCF7 cell lysates; PC-12 and L6 cell lysates; Rat liver, brain, testis and kidney tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa. |
Target
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Function
Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. -
Tissue specificity
Widely expressed, enriched in testis compared to other tissues. -
Sequence similarities
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2289 Human
- Omim: 602623 Human
- SwissProt: Q13451 Human
- Unigene: 407190 Human
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Alternative names
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
see all
Images
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Western blot - Anti-FKBP51 antibody (ab2901)All lanes : Anti-FKBP51 antibody (ab2901) at 2 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear enriched extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) nuclear enriched extract
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) nuclear enriched extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) nuclear enriched extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/2500 dilutionApproximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested
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Western blot - Anti-FKBP51 antibody (ab2901)All lanes : Anti-FKBP51 antibody (ab2901) at 1 µg/ml
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : L6 (Rat skeletal muscle cell line) whole cell lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat testis tissue lysate
Lane 6 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.Western blot was performed using ab2901 and a 51kDa band corresponding to FKBP5 was observed across all the tested cell lines and tissues. Whole cell extracts were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel . Resolved proteins were then transferred onto a Nitrocellulose membrane by iBlot® 2 Dry Blotting System. The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP using the iBright FL 1000. Chemiluminescent detection was performed using Novex® ECL Reagent Kit.
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Western blot - Anti-FKBP51 antibody (ab2901)Anti-FKBP51 antibody (ab2901) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab2901 has been referenced in 18 publications.
- Gao Z et al. FK506-binding protein 5 promotes the progression of papillary thyroid carcinoma. J Int Med Res 49:3000605211008325 (2021). PubMed: 33906532
- Corbin JM et al. Seed-mediated RNA interference of androgen signaling and survival networks induces cell death in prostate cancer cells. Mol Ther Nucleic Acids 24:337-351 (2021). PubMed: 33850637
- Zhang C et al. The deficiency of FKBP-5 inhibited hepatocellular progression by increasing the infiltration of distinct immune cells and inhibiting obesity-associated gut microbial metabolite. J Gastrointest Oncol 12:711-721 (2021). PubMed: 34012660
- Lu A et al. Heterogenetic parabiosis between healthy and dystrophic mice improve the histopathology in muscular dystrophy. Sci Rep 10:7075 (2020). PubMed: 32341395
- Hadamitzky M et al. Repeated Systemic Treatment with Rapamycin Affects Behavior and Amygdala Protein Expression in Rats. Int J Neuropsychopharmacol 21:592-602 (2018). PubMed: 29462337