Anti-FKBP51 antibody (ab2901)
Key features and details
- Rabbit polyclonal to FKBP51
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FKBP51 antibody
See all FKBP51 primary antibodies -
Description
Rabbit polyclonal to FKBP51 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa. By Western blot, this antibody detects an ~51 kDa protein representing FKBP 51 in HeLa cell lysate.
By Western blot this antibody also detects FKBP 51 in whole tissue extracts from rat kidney and rat and mouse testes.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa. By Western blot, this antibody detects an ~51 kDa protein representing FKBP 51 in HeLa cell lysate. By Western blot this antibody also detects FKBP 51 in whole tissue extracts from rat kidney and rat and mouse testes. |
Target
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Function
Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. -
Tissue specificity
Widely expressed, enriched in testis compared to other tissues. -
Sequence similarities
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2289 Human
- Omim: 602623 Human
- SwissProt: Q13451 Human
- Unigene: 407190 Human
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Alternative names
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
see all
Images
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All lanes : Anti-FKBP51 antibody (ab2901) at 2 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear enriched extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) nuclear enriched extract
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) nuclear enriched extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) nuclear enriched extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/2500 dilutionApproximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested
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Anti-FKBP51 antibody (ab2901) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Protocols
References (15)
ab2901 has been referenced in 15 publications.
- Lu A et al. Heterogenetic parabiosis between healthy and dystrophic mice improve the histopathology in muscular dystrophy. Sci Rep 10:7075 (2020). PubMed: 32341395
- Hadamitzky M et al. Repeated Systemic Treatment with Rapamycin Affects Behavior and Amygdala Protein Expression in Rats. Int J Neuropsychopharmacol 21:592-602 (2018). PubMed: 29462337
- Criado-Marrero M et al. Dynamic expression of FKBP5 in the medial prefrontal cortex regulates resiliency to conditioned fear. Learn Mem 24:145-152 (2017). PubMed: 28298552
- Li K et al. ELF5-Mediated AR Activation Regulates Prostate Cancer Progression. Sci Rep 7:42759 (2017). PubMed: 28287091
- Herring A et al. Kallikrein-8 inhibition attenuates Alzheimer's disease pathology in mice. Alzheimers Dement 12:1273-1287 (2016). PubMed: 27327541