- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-FKBP51 antibody
See all FKBP51 primary antibodies -
DescriptionRabbit polyclonal to FKBP51
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Hamster, Dog, Human, Xenopus laevis, Non human primates
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Immunogen
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Positive control
- WB: HeLa cell lysate. K562, HepG2, PC-3 and MCF7 nuclear enriched extracts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab2901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa.Can be blocked with FKBP51 peptide (ab4924). By Western blot, this antibody detects an ~51 kDa protein representing FKBP 51 in HeLa cell lysate. By Western blot this antibody also detects FKBP 51 in whole tissue extracts from rat kidney and rat and mouse testes. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 20422297 |
Target
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FunctionInteracts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
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Tissue specificityWidely expressed, enriched in testis compared to other tissues.
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Sequence similaritiesContains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 481759 Dog
- Entrez Gene: 2289 Human
- Entrez Gene: 14229 Mouse
- Entrez Gene: 361810 Rat
- Omim: 602623 Human
- SwissProt: Q13451 Human
- SwissProt: Q64378 Mouse
- Unigene: 407190 Human
see all -
Alternative names
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
see all
Images
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Western blot - Anti-FKBP51 antibody (ab2901)All lanes : Anti-FKBP51 antibody (ab2901) at 2 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear enriched extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) nuclear enriched extract
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) nuclear enriched extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) nuclear enriched extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/2500 dilutionApproximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested
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Western blot - Anti-FKBP51 antibody (ab2901)Anti-FKBP51 antibody (ab2901) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Protocols
References
This product has been referenced in:
- Li K et al. ELF5-Mediated AR Activation Regulates Prostate Cancer Progression. Sci Rep 7:42759 (2017). Read more (PubMed: 28287091) »
- Allan AM et al. Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning. PLoS One 9:e96200 (2014). WB ; Mouse . Read more (PubMed: 24755652) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"