• Product name
  • Description
    Rabbit polyclonal to FKBP51
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human, Xenopus laevis, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human FKBP51 aa 394-407.


    Database link: Q13451
    (Peptide available as ab4924)

  • Positive control
    • WB: HeLa whole cell lysate (ab150035). K562, HepG2, PC-3 and MCF7 nuclear enriched extracts.



Our Abpromise guarantee covers the use of ab2901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa.Can be blocked with FKBP51 peptide (ab4924). By Western blot, this antibody detects an ~51 kDa protein representing FKBP 51 in HeLa cell lysate. By Western blot this antibody also detects FKBP 51 in whole tissue extracts from rat kidney and rat and mouse testes.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20422297


  • Function
    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • Tissue specificity
    Widely expressed, enriched in testis compared to other tissues.
  • Sequence similarities
    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 51 kDa FK506 binding protein 5 antibody
    • 51 kDa FK506 binding protein antibody
    • 51 kDa FK506-binding protein antibody
    • 51 kDa FKBP antibody
    • 54 kDa progesterone receptor associated immunophilin antibody
    • 54 kDa progesterone receptor-associated immunophilin antibody
    • AIG 6 antibody
    • AIG6 antibody
    • Androgen regulated protein 6 antibody
    • Androgen-regulated protein 6 antibody
    • FF1 antigen antibody
    • FK506 binding protein 5 antibody
    • FK506-binding protein 5 antibody
    • FKBP 5 antibody
    • FKBP 51 antibody
    • FKBP 54 antibody
    • FKBP-5 antibody
    • FKBP-51 antibody
    • FKBP5 antibody
    • FKBP5_HUMAN antibody
    • FKBP54 antibody
    • HSP90 binding immunophilin antibody
    • HSP90-binding immunophilin antibody
    • MGC111006 antibody
    • OTTHUMP00000016268 antibody
    • P54 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP5 antibody
    • Ptg 10 antibody
    • Ptg10 antibody
    • Rotamase antibody
    • T cell FK506 binding protein antibody
    see all


  • All lanes : Anti-FKBP51 antibody (ab2901) at 2 µg/ml

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear enriched extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) nuclear enriched extract
    Lane 3 : PC-3 (human prostate adenocarcinoma cell line) nuclear enriched extract
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) nuclear enriched extract

    Lysates/proteins at 30 µg per lane.

    All lanes : Goat anti-Rabbit IgG (H+L) at 1/2500 dilution

    Approximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested

  • Anti-FKBP51 antibody (ab2901) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate


This product has been referenced in:
  • Hadamitzky M  et al. Repeated Systemic Treatment with Rapamycin Affects Behavior and Amygdala Protein Expression in Rats. Int J Neuropsychopharmacol 21:592-602 (2018). Read more (PubMed: 29462337) »
  • Criado-Marrero M  et al. Dynamic expression of FKBP5 in the medial prefrontal cortex regulates resiliency to conditioned fear. Learn Mem 24:145-152 (2017). Read more (PubMed: 28298552) »
See all 12 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (293Tcells)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
20 µg
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 28 2016


Merci pour votre enquête.

A ma connaissance, il n'existent pas des isoformes décrites de FKBP51. Il existent par contre des sites de phosphorylation. Un doublet de la bande autour de 50kD pourrait donc être du à phosphorylation différentielle. Je ne sais par contre pas à quoi les bandes entre 15 et 20kD pourraient correspondre.

Pour exclure que les bandes observées sont des bandes non-spécifiques, je peux vous proposer d'investiguer les points suivants:

1.) Les bandes pourraient être du bruit de fond du à l'anticorps secondaire reconnaissante des immunoglobulines endogènes ou du milieu de culture. Pour exclure cette possibilité, il suffit de faire à contrôle ou vous suivez le même protocole avec les mêmes échantillons etomettez l'anticorps primaire.

2.) Les bandes observées entre 15 et 20kD pourraient être des produits de dégradation. Est-ce que vous avez ajouté des inhibiteurs de protéases au lysats? Je peux conseiller de préparer les lysats frais en ajoutant des inhibiteurs de protéases.

Je serais ravi d'essayer de donner plus de conseils. Pour ceci je devrais regarder vos résultats en plus de détails. Si vous souhaitez ceci, veuillez me faire parvenirle plus d'informations possible sur le protocole que vous avez utilisé avec l'aide du questionnaire ci-joint. Celui-ci nous permettra de vous fournir la meilleure assistance technique.

Je vous remercie par avance de nous envoyer votre protocole.

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Western blot
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted May 27 2011


Thank you for your enquiry. We did a BLAST search on the immunizing sequence and the only FKBP that came up was 51. We do not think this is 12 but it may be a degraded fragment from 51.

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