Recombinant Anti-FKBP51 antibody [EPR6617] (ab126715)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6617] to FKBP51
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FKBP51 antibody [EPR6617]
See all FKBP51 primary antibodies -
Description
Rabbit monoclonal [EPR6617] to FKBP51 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon, Rat stomach and Human prostatic hyperplasia tissue. WB: Human testis tissue; Jurkat, HepG2, Caco-2 and HeLa whole cell lysate (ab150035). Wild-type HAP1 whole cell lysate. ICC/IF: Jurkat cells. Flow Cyt (intra): HeLa cells. IP: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6617 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. -
Tissue specificity
Widely expressed, enriched in testis compared to other tissues. -
Sequence similarities
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2289 Human
- Entrez Gene: 361810 Rat
- Omim: 602623 Human
- SwissProt: Q13451 Human
- Unigene: 407190 Human
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Alternative names
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab126715 observed at 51 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab126715 was shown to recognize FKBP51 in wild-type cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. Ab126715 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-FKBP51 antibody [EPR6617] (ab126715) at 1/10000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg
Lane 2 : Rat spleen lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
ab126715 (purified) at 1:20 dilution (2µg) immunoprecipitating FKBP51 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab126715 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126715 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling FKBP51 with purified ab126715 at 1:250 dilution (1.156 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling FKBP51 with purified ab126715 at 1:250 dilution (1.156 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling FKBP51 with purified ab126715 at 1:100 dilution (2.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Alexa Fluor® 488 (ab198978) and Alexa Fluor® 647 (ab198979) conjugated versions are available for this clone.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FKBP51 with purified ab126715 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Alexa Fluorr®488 (ab198978) and Alexa Fluorr®647 (ab198979) conjugated versions are available for this clone.
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All lanes : Anti-FKBP51 antibody [EPR6617] (ab126715) at 1/1000 dilution
Lane 1 : Human testis cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 51 kDa -
ab126715, at 1/50 dilution, stains FKBP51 in paraffin embedded human colon tissue by immunohistochemistry.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab126715 has been referenced in 9 publications.
- Maeda K et al. FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells. Mol Oncol 16:940-956 (2022). PubMed: 34057812
- Habara M et al. FKBP52 and FKBP51 differentially regulate the stability of estrogen receptor in breast cancer. Proc Natl Acad Sci U S A 119:e2110256119 (2022). PubMed: 35394865
- Chen Y et al. FKBP51 induces p53-dependent apoptosis and enhances drug sensitivity of human non-small-cell lung cancer cells. Exp Ther Med 19:2236-2242 (2020). PubMed: 32104289
- Hao W et al. FKBP5 Regulates RIG-I-Mediated NF-?B Activation and Influenza A Virus Infection. Viruses 12:N/A (2020). PubMed: 32580383
- Kamble PG et al. Proof-of-concept for CRISPR/Cas9 gene editing in human preadipocytes: Deletion of FKBP5 and PPARG and effects on adipocyte differentiation and metabolism. Sci Rep 10:10565 (2020). PubMed: 32601291
- Li M et al. A Non-canonical Pathway with Potential for Safer Modulation of Transforming Growth Factor-ß1 in Steroid-Resistant Airway Diseases. iScience 12:232-246 (2019). PubMed: 30711747
- Sidibeh CO et al. FKBP5 expression in human adipose tissue: potential role in glucose and lipid metabolism, adipogenesis and type 2 diabetes. Endocrine 62:116-128 (2018). PubMed: 30032404
- Takaoka M et al. FKBP51 regulates cell motility and invasion via RhoA signaling. Cancer Sci 108:380-389 (2017). PubMed: 28032931
- Kashiwagi E et al. EP2 signaling mediates suppressive effects of celecoxib on androgen receptor expression and cell proliferation in prostate cancer. Prostate Cancer Prostatic Dis 17:10-7 (2014). PubMed: 24518515