Key features and details
- Mouse monoclonal [Hi51B] to FKBP51
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human
- Isotype: IgG
Product nameAnti-FKBP51 antibody [Hi51B]
See all FKBP51 primary antibodies
DescriptionMouse monoclonal [Hi51B] to FKBP51
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human
Full length Human FKBP51, binding epitope in TPR domain
- WB: HeLa whole cell lysate (ab150035), rat kidney and rat and mouse testes tissue lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 51 kDa.|
FunctionInteracts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
Tissue specificityWidely expressed, enriched in testis compared to other tissues.
Sequence similaritiesContains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 51 kDa FK506 binding protein 5 antibody
- 51 kDa FK506 binding protein antibody
- 51 kDa FK506-binding protein antibody
ab79844 at 1/100 dilution staining FKBP51 in normal mouse MK cells (IF localization shown in red) by Immunocytochemistry/ immunofluorescence.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab79844 observed at 51 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab79844 was shown to recognize FKBP51 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. ab79844 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab79844 has not yet been referenced specifically in any publications.