Product nameAnti-FKBP52 antibody
See all FKBP52 primary antibodies
DescriptionRabbit polyclonal to FKBP52
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human FKBP52 aa 400-440. The exact sequence is proprietary.
Database link: Q02790
- WB: HeLa and HEK-293T whole cell lysate; IP: HeLa whole cell lysate; IHC-P: Human prostate carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab264204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/2000 - 1/10000.|
FunctionImmunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
Tissue specificityWidely expressed.
Sequence similaritiesContains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats.
DomainThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
modificationsPhosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
- Information by UniProt
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
All lanes : Anti-FKBP52 antibody (ab264204) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Detected with chemilimuniscence with exposure time of 30 seconds.
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
FKBP52 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264204 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264204 at 1 µg/ml.
Lane 1: ab264204 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling FKBP52 with ab264204 at 1/1,000 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264204 has not yet been referenced specifically in any publications.