Overview

  • Product name

    Anti-FKBP52 antibody [EPR21120]
    See all FKBP52 primary antibodies
  • Description

    Rabbit monoclonal [EPR21120] to FKBP52
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FKBP52 aa 200-300. The exact sequence is proprietary.
    Database link: Q02790

  • Positive control

    • WB: K562, MCF7, HeLa, HEK-293T and RAW 264.7 whole cell lysate. Mouse and rat brain lysate. Human fetal kidney lysate. IHC-P: Human breast cancer tissue. Mouse and rat cerebrum tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab230952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Use at a 1:4,000 dilution for human samples and a 1:1,000 for mouse and rat samples.

Target

  • Function

    Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Domain

    The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • Post-translational
    modifications

    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 51 kDa FK506-binding protein antibody
    • 52 kDa FK506 binding protein antibody
    • 52 kDa FK506-binding protein antibody
    • 52 kDa FKBP antibody
    • 59 kDa immunophilin antibody
    • FK506 binding protein 4 antibody
    • FK506-binding protein 4 antibody
    • FKBP 4 antibody
    • FKBP 52 antibody
    • FKBP 59 antibody
    • FKBP-4 antibody
    • FKBP-52 antibody
    • FKBP4 antibody
    • FKBP4_HUMAN antibody
    • FKBP51 antibody
    • FKBP52 protein antibody
    • FKBP59 antibody
    • HBI antibody
    • Hsp 56 antibody
    • HSP binding immunophilin antibody
    • HSP-binding immunophilin antibody
    • Hsp56 antibody
    • Immunophilin FKBP52 antibody
    • N-terminally processed antibody
    • p52 antibody
    • p59 antibody
    • p59 protein antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • peptidyl-prolyl cis-trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP4 antibody
    • Rotamase antibody
    • T cell FK506 binding protein 59kD antibody
    see all

Images

  • Lanes 1-3 & 7 : Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/5000 dilution
    Lanes 4-6 & 8 : Anti-FKBP52 antibody [EPR21120] (ab230952) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
    Lane 5 : Mouse brain lysate at 10 µg
    Lane 6 : Rat brain lysate at 10 µg
    Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 8 : Human fetal kidney lysate at 10 µg

    Secondary
    Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 8 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 52 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 81 seconds; Lane 4: 30 second; Lanes 5: 120 seconds; Lane 6-8: 3 minutes.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 26065228).

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling FKBP52 with ab230952 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in human breast cancer (PMID: 19584157) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling FKBP52 with ab230952 at 1/1,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in rat cerebrum (PMID: 20133804; PMID: 20422297) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling FKBP52 with ab230952 at 1/1,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in mouse cerebrum (PMID: 20133804; PMID: 20422297) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab230952 has not yet been referenced specifically in any publications.

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