Overview

  • Product name

    Anti-FKBP52 antibody [EPR21125]
    See all FKBP52 primary antibodies
  • Description

    Rabbit monoclonal [EPR21125] to FKBP52
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FKBP52 aa 1-300. The exact sequence is proprietary.
    Database link: Q02790

  • Positive control

    • WB: K562, HeLa and HEK-293T cell lysate; Human fetal brain lysate. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells. IP: HeLa cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab230951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF 1/100.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Domain

    The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • Post-translational
    modifications

    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 51 kDa FK506-binding protein antibody
    • 52 kDa FK506 binding protein antibody
    • 52 kDa FK506-binding protein antibody
    • 52 kDa FKBP antibody
    • 59 kDa immunophilin antibody
    • FK506 binding protein 4 antibody
    • FK506-binding protein 4 antibody
    • FKBP 4 antibody
    • FKBP 52 antibody
    • FKBP 59 antibody
    • FKBP-4 antibody
    • FKBP-52 antibody
    • FKBP4 antibody
    • FKBP4_HUMAN antibody
    • FKBP51 antibody
    • FKBP52 protein antibody
    • FKBP59 antibody
    • HBI antibody
    • Hsp 56 antibody
    • HSP binding immunophilin antibody
    • HSP-binding immunophilin antibody
    • Hsp56 antibody
    • Immunophilin FKBP52 antibody
    • N-terminally processed antibody
    • p52 antibody
    • p59 antibody
    • p59 protein antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • peptidyl-prolyl cis-trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP4 antibody
    • Rotamase antibody
    • T cell FK506 binding protein 59kD antibody
    see all

Images

  • Lanes 1-2 : Anti-FKBP52 antibody [EPR21125] (ab230951) at 1/5000 dilution
    Lanes 3-4 : Anti-FKBP52 antibody [EPR21125] (ab230951) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
    Lane 4 : Human fetal brain lysate at 10 µg

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-2: 48 seconds; Lane 3: 30 seconds; Lane 4: 3 minutes.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 26065228).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling FKBP52 with ab230951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line. 

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue). 

    Secondary antibody only control: Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • FKBP52 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab230951 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab230951 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230951 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling FKBP52 with ab230951 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in MCF7 cell line.

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling FKBP52 with ab230951 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

References

ab230951 has not yet been referenced specifically in any publications.

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