RecombinantRabMAb

Recombinant Anti-FKBP52 antibody [EPR6618] (ab129097)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6618] to FKBP52
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FKBP52 antibody [EPR6618]
    See all FKBP52 primary antibodies

  • Description

    Rabbit monoclonal [EPR6618] to FKBP52

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab129097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Contains 2 PPIase FKBP-type domains.
      Contains 3 TPR repeats.

    • Domain

      The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
      The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
      The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.

    • Post-translational
      modifications

      Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 51 kDa FK506-binding protein antibody
      • 52 kDa FK506 binding protein antibody
      • 52 kDa FK506-binding protein antibody
      • 52 kDa FKBP antibody
      • 59 kDa immunophilin antibody
      • FK506 binding protein 4 antibody
      • FK506-binding protein 4 antibody
      • FKBP 4 antibody
      • FKBP 52 antibody
      • FKBP 59 antibody
      • FKBP-4 antibody
      • FKBP-52 antibody
      • FKBP4 antibody
      • FKBP4_HUMAN antibody
      • FKBP51 antibody
      • FKBP52 protein antibody
      • FKBP59 antibody
      • HBI antibody
      • Hsp 56 antibody
      • HSP binding immunophilin antibody
      • HSP-binding immunophilin antibody
      • Hsp56 antibody
      • Immunophilin FKBP52 antibody
      • N-terminally processed antibody
      • p52 antibody
      • p59 antibody
      • p59 protein antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • peptidyl-prolyl cis-trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
      • Peptidylprolyl cis trans isomerase antibody
      • PPIase antibody
      • PPIase FKBP4 antibody
      • Rotamase antibody
      • T cell FK506 binding protein 59kD antibody
      see all

    Images

    • Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)
      All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : K562 cell lysates
      Lane 4 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)

      ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)

      ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)

      ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097)
      OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-FKBP52 antibody [EPR6618] (ab129097)
      Anti-FKBP52 antibody [EPR6618] (ab129097)

    References (5)

    Publishing research using ab129097? Please let us know so that we can cite the reference in this datasheet.

    ab129097 has been referenced in 5 publications.

    • Lin CC  et al. Effects of early life social experience on fear extinction and related glucocorticoid profiles - behavioral and neurochemical approaches in a rat model of PTSD. Behav Brain Res 391:112686 (2020). PubMed: 32428628
    • Song S & Tan Y Expression of FKBP52 in the ovaries of PCOS rats. Int J Mol Med 43:868-878 (2019). PubMed: 30483787
    • Li YE  et al. Identification of high-confidence RNA regulatory elements by combinatorial classification of RNA-protein binding sites. Genome Biol 18:169 (2017). PubMed: 28886744
    • Cao G  et al. Xiao Yao San against Corticosterone-Induced Stress Injury via Upregulating Glucocorticoid Receptor Reaction Element Transcriptional Activity. Evid Based Complement Alternat Med 2016:5850739 (2016). PubMed: 27822288
    • Caldwell KK  et al. The impact of prenatal alcohol exposure on hippocampal-dependent outcome measures is influenced by prenatal and early-life rearing conditions. Alcohol Clin Exp Res 39:631-9 (2015). PubMed: 25752869

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