Recombinant
RabMAb

Recombinant Anti-FKBP52 antibody [EPR6618] (ab129097)

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Overview

  • Product name

    Anti-FKBP52 antibody [EPR6618]
    See all FKBP52 primary antibodies

  • Description

    Rabbit monoclonal [EPR6618] to FKBP52

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab129097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Contains 2 PPIase FKBP-type domains.
      Contains 3 TPR repeats.

    • Domain

      The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
      The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
      The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.

    • Post-translational
      modifications

      Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 51 kDa FK506-binding protein antibody
      • 52 kDa FK506 binding protein antibody
      • 52 kDa FK506-binding protein antibody
      • 52 kDa FKBP antibody
      • 59 kDa immunophilin antibody
      • FK506 binding protein 4 antibody
      • FK506-binding protein 4 antibody
      • FKBP 4 antibody
      • FKBP 52 antibody
      • FKBP 59 antibody
      • FKBP-4 antibody
      • FKBP-52 antibody
      • FKBP4 antibody
      • FKBP4_HUMAN antibody
      • FKBP51 antibody
      • FKBP52 protein antibody
      • FKBP59 antibody
      • HBI antibody
      • Hsp 56 antibody
      • HSP binding immunophilin antibody
      • HSP-binding immunophilin antibody
      • Hsp56 antibody
      • Immunophilin FKBP52 antibody
      • N-terminally processed antibody
      • p52 antibody
      • p59 antibody
      • p59 protein antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • peptidyl-prolyl cis-trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
      • Peptidylprolyl cis trans isomerase antibody
      • PPIase antibody
      • PPIase FKBP4 antibody
      • Rotamase antibody
      • T cell FK506 binding protein 59kD antibody
      see all

    Images

    • Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)
      All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : K562 cell lysates
      Lane 4 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)

      ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)

      ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)

      ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Other - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Other - Anti-FKBP52 antibody [EPR6618] (ab129097)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 4 Publications for this product

    Publishing research using ab129097? Please let us know so that we can cite the reference in this datasheet.

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