Recombinant
RabMAb

Recombinant Anti-FKBP52 antibody [EPR6618] (Alexa Fluor® 647) (ab202766)

Overview

  • Product name

    Anti-FKBP52 antibody [EPR6618] (Alexa Fluor® 647)
    See all FKBP52 primary antibodies
  • Description

    Rabbit monoclonal [EPR6618] to FKBP52 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Applications

Our Abpromise guarantee covers the use of ab202766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

Flow Cyt 1/500.

Target

  • Function

    Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Domain

    The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • Post-translational
    modifications

    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 51 kDa FK506-binding protein antibody
    • 52 kDa FK506 binding protein antibody
    • 52 kDa FK506-binding protein antibody
    • 52 kDa FKBP antibody
    • 59 kDa immunophilin antibody
    • FK506 binding protein 4 antibody
    • FK506-binding protein 4 antibody
    • FKBP 4 antibody
    • FKBP 52 antibody
    • FKBP 59 antibody
    • FKBP-4 antibody
    • FKBP-52 antibody
    • FKBP4 antibody
    • FKBP4_HUMAN antibody
    • FKBP51 antibody
    • FKBP52 protein antibody
    • FKBP59 antibody
    • HBI antibody
    • Hsp 56 antibody
    • HSP binding immunophilin antibody
    • HSP-binding immunophilin antibody
    • Hsp56 antibody
    • Immunophilin FKBP52 antibody
    • N-terminally processed antibody
    • p52 antibody
    • p59 antibody
    • p59 protein antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • peptidyl-prolyl cis-trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP4 antibody
    • Rotamase antibody
    • T cell FK506 binding protein 59kD antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab202766 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab202766, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) /permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • ab202766 staining FKBP52 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202766 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab202766 has not yet been referenced specifically in any publications.

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