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Synthetic peptide corresponding to human FKBP52
Our Abpromise guarantee covers the use of ab59460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Paraffin-embedded mouse backskin tissue stained for FKBP52 using ab59460 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.
ab59460 at 1/100 dilution staining FKBP52 in prostate tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti mouse secondary was used at 1/10 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"