Key features and details
- Mouse monoclonal [Hi52C] to FKBP52
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Hamster, Dog, Human
- Isotype: IgG1
Product nameAnti-FKBP52 antibody [Hi52C]
See all FKBP52 primary antibodies
DescriptionMouse monoclonal [Hi52C] to FKBP52
Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Dog, Human
Synthetic peptide corresponding to human FKBP52
- IHC-P: Human prostate tissue; Mouse backskin tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab59460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionImmunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
Tissue specificityWidely expressed.
Sequence similaritiesContains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats.
DomainThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
modificationsPhosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
- Information by UniProt
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
ICC/IF image of ab59460 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59460, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Paraffin-embedded mouse backskin tissue stained for FKBP52 using ab59460 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.
ab59460 at 1/100 dilution staining FKBP52 in prostate tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti mouse secondary was used at 1/10 dilution.
Overlay histogram showing HeLa cells stained with ab59460 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59460, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab59460 has been referenced in 3 publications.
- Hong Y et al. Amelioration of muscle wasting by glucagon-like peptide-1 receptor agonist in muscle atrophy. J Cachexia Sarcopenia Muscle N/A:N/A (2019). PubMed: 31020810
- Harrell CS et al. Pharmacological stimulation of hypoxia inducible factor-1a facilitates the corticosterone response to a mild acute stressor. Neurosci Lett 600:75-9 (2015). PubMed: 26037418
- Pare JM et al. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol Biol Cell 24:2303-10 (2013). PubMed: 23741051