Overview

  • Product name

    Anti-FKBP6 antibody [EPR3673]
    See all FKBP6 primary antibodies
  • Description

    Rabbit monoclonal [EPR3673] to FKBP6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FKBP6 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Recombinant human FKBP6 protein (ab95497) can be used as a positive control in WB. Molt-4, Saos-2, 293T and Human heart lysates; Human breast tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3673
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      PPIases accelerate the folding of proteins.
    • Tissue specificity

      Detected in all tissues examined, with higher expression in testis, heart, skeletal muscle, liver, and kidney.
    • Involvement in disease

      Note=FKBP6 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of FKBP6 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities

      Contains 1 PPIase FKBP-type domain.
      Contains 3 TPR repeats.
    • Information by UniProt
    • Database links

    • Alternative names

      • 36 kDa FK506 binding protein antibody
      • 36 kDa FK506-binding protein antibody
      • 36 kDa FKBP antibody
      • EC 5.2.1.8 antibody
      • FK506 binding protein 6 antibody
      • FK506-binding protein 6 antibody
      • FKBP 36 antibody
      • FKBP-36 antibody
      • FKBP-6 antibody
      • FKBP36 antibody
      • fkbp6 antibody
      • FKBP6_HUMAN antibody
      • Immunophilin FKBP36 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP6 antibody
      • PPIase antibody
      • PPIase FKBP6 antibody
      • Rotamase antibody
      see all

    Images

    • All lanes : Anti-FKBP6 antibody [EPR3673] (ab108419) at 1/50000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 38 kDa

    • ab108419, at 1/100 dilution, staining FKBP6 in paraffin-embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Zhu Z  et al. Rapamycin-mediated mTOR inhibition impairs silencing of sex chromosomes and the pachytene piRNA pathway in the mouse testis. Aging (Albany NY) 11:185-208 (2019). Read more (PubMed: 30636722) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer


    I have issued a free of charge replacement with the order number **** for ab92475.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Answer

    Thank you for the WB details. I see nothing wrong with your protocol. Loading more sample might help but you should see something with 10ug of this lysate.

    We do not have a different lot of this antibody and I am concerned that another vial of the same lot will give the same result. Would you be interested in trying a different rabbit monoclonal, raised against the C terminus of FKBP6? The catalogue number is ab92475:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=92475).

    Read More

    Answer

    Thank you for bringing this to our attention.

    Can you please provide a few details of your protocol? If there are no suggestions we can make, then a replacement will not be a problem.

    How are the 293T lysates prepared? Do you include protease inhibitors?

    How much protein did you load per lane of the gel?

    Are you confident that the transfer was successful?

    How is the membrane blocked?

    How much antibody (concentration or dilution) is applied to the blot and for how long?

    Are you confident the secondary antibody is effective?

    I look forward to your reply.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up