Key features and details
- Rabbit polyclonal to FKSG14/p33
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FKSG14/p33 antibody
DescriptionRabbit polyclonal to FKSG14/p33
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human FKSG14/p33 aa 1-269.
MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETL TDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKL RQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKV ETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKK NIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNG IALRHPEDPTRIRLEAFHQ
Database link: Q9BS16
- WB: PC-3 and HEK-293T whole cell lysates. IHC-P: Human skin and liver tissue.
Previously labelled as FKSG14.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab236739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 32 kDa.|
RelevanceFKSG14 / p33, also known as CENPK (centromere protein K) is a component of the CENPA-CAD (nucleosome distal) complex. It may be involved in incorporation of CENPA into centromeres and is required for proper kinetochore function, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. FKSG14 / p33 constitutively localized to centromeres throughout the cell cycle. There are 3 isoforms produced by alternative splicing.
Cellular localizationNuclear. Centromere. Kinetochore. Localizes exclusively in the centromeres.
- AF5 alpha antibody
- AF5alpha antibody
- B130045K24Rik antibody
All lanes : Anti-FKSG14/p33 antibody (ab236739) at 1/1000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32 kDa
Paraffin-embedded human skin tissue stained for FKSG14/p33 using ab236739 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitating FKSG14/p33 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ab236739 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ab236739 (8μg) + Hela whole cell lysate (500μg)
Lane 3: HeLa whole cell lysate (10μg)
Paraffin-embedded human liver tissue stained for FKSG14/p33 using ab236739 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236739 has not yet been referenced specifically in any publications.