Anti-FKSG14/p33 antibody (ab236739)
Key features and details
- Rabbit polyclonal to FKSG14/p33
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FKSG14/p33 antibody -
Description
Rabbit polyclonal to FKSG14/p33 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human FKSG14/p33 aa 1 to the C-terminus.
Database link: Q9BS16 -
Positive control
- WB: PC-3 and HEK-293T whole cell lysates. IHC-P: Human skin and liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 32 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 32 kDa. |
Target
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Relevance
FKSG14 / p33, also known as CENPK (centromere protein K) is a component of the CENPA-CAD (nucleosome distal) complex. It may be involved in incorporation of CENPA into centromeres and is required for proper kinetochore function, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. FKSG14 / p33 constitutively localized to centromeres throughout the cell cycle. There are 3 isoforms produced by alternative splicing. -
Cellular localization
Nuclear. Centromere. Kinetochore. Localizes exclusively in the centromeres. -
Database links
- Entrez Gene: 64105 Human
- Omim: 611502 Human
- SwissProt: Q9BS16 Human
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Alternative names
- AF5 alpha antibody
- AF5alpha antibody
- B130045K24Rik antibody
see all
Images
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All lanes : Anti-FKSG14/p33 antibody (ab236739) at 1/1000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32 kDa -
Paraffin-embedded human skin tissue stained for FKSG14/p33 using ab236739 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitating FKSG14/p33 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ab236739 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ab236739 (8μg) + Hela whole cell lysate (500μg)
Lane 3: HeLa whole cell lysate (10μg) -
Paraffin-embedded human liver tissue stained for FKSG14/p33 using ab236739 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236739 has been referenced in 1 publication.
- Wang Y et al. Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion. Adv Clin Exp Med 30:691-699 (2021). PubMed: 34118147