Anti-FLAP antibody (ab85227)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-FLAP antibody
See all FLAP primary antibodies -
Description
Rabbit polyclonal to FLAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Cow, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human FLAP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab98291) -
Positive control
- This antibody gave a positive signal in Human small intestine tissue lysate as well as the following whole cell lysates: U937; THP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab85227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. -
Involvement in disease
Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. -
Sequence similarities
Belongs to the MAPEG family. -
Domain
The C-terminal part after residue 140 is mostly unstructured. -
Cellular localization
Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 100034178 Horse
- Entrez Gene: 241 Human
- Entrez Gene: 11690 Mouse
- Entrez Gene: 100009413 Rabbit
- Omim: 603700 Human
- SwissProt: P30353 Horse
- SwissProt: P20292 Human
- SwissProt: P30355 Mouse
see all -
Alternative names
- 5-lipoxygenase activating protein antibody
- AL5AP_HUMAN antibody
- ALOX 5AP antibody
see all
Images
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Western blot - Anti-FLAP antibody (ab85227)All lanes : Anti-FLAP antibody (ab85227) at 1 µg/ml
Lane 1 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 175 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab85227)ICC/IF image of ab85227 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References
This product has been referenced in:
- Werner M et al. Targeting biosynthetic networks of the proinflammatory and proresolving lipid metabolome. FASEB J 33:6140-6153 (2019). Read more (PubMed: 30735438) »
- Rao Z et al. Vacuolar (H+)-ATPase Critically Regulates Specialized Proresolving Mediator Pathways in Human M2-like Monocyte-Derived Macrophages and Has a Crucial Role in Resolution of Inflammation. J Immunol 203:1031-1043 (2019). Read more (PubMed: 31300512) »
