Anti-FLAP antibody (ab85227)

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Overview

  • Product name
    Anti-FLAP antibody
    See all FLAP primary antibodies
  • Description
    Rabbit polyclonal to FLAP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FLAP.

    Read Abcam's proprietary immunogen policy (Peptide available as ab98291.)

  • Positive control
    • This antibody gave a positive signal in Human small intestine tissue lysate as well as the following whole cell lysates: U937; THP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab85227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
  • Involvement in disease
    Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition.
  • Sequence similarities
    Belongs to the MAPEG family.
  • Domain
    The C-terminal part after residue 140 is mostly unstructured.
  • Cellular localization
    Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 5-lipoxygenase activating protein antibody
    • AL5AP_HUMAN antibody
    • ALOX 5AP antibody
    • ALOX5 AP antibody
    • ALOX5AP antibody
    • Arachidonate 5 lipoxygenase activating protein antibody
    • Arachidonate 5-lipoxygenase-activating protein antibody
    • Five lipoxygenase activating protein antibody
    • FLAP antibody
    • MK 886 binding protein antibody
    • MK-886-binding protein antibody
    see all

Images

  • Western blot - Anti-FLAP antibody (ab85227)
    Western blot - Anti-FLAP antibody (ab85227)
    All lanes : Anti-FLAP antibody (ab85227) at 1 µg/ml

    Lane 1 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa
    Additional bands at: 175 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab85227)
    Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab85227)
    ICC/IF image of ab85227 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Werz O  et al. Human macrophages differentially produce specific resolvin or leukotriene signals that depend on bacterial pathogenicity. Nat Commun 9:59 (2018). WB, IF ; Human . Read more (PubMed: 29302056) »
  • Garscha U  et al. Pharmacological profile and efficiency in vivo of diflapolin, the first dual inhibitor of 5-lipoxygenase-activating protein and soluble epoxide hydrolase. Sci Rep 7:9398 (2017). ICC/IF . Read more (PubMed: 28839250) »
See all 4 Publications for this product

Publishing research using ab85227? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-FLAP antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse bone marrow derived mast cells)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
1.5e+006 cells
Specification
mouse bone marrow derived mast cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Read More

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Submitted Jun 09 2016

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