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    flap-antibody-ab85227.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-FLAP antibody (ab85227)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

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Western blot - Anti-FLAP antibody (ab85227)
  • Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab85227)

Key features and details

  • Rabbit polyclonal to FLAP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FLAP antibody
    See all FLAP primary antibodies
  • Description

    Rabbit polyclonal to FLAP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in Human small intestine tissue lysate as well as the following whole cell lysates: U937; THP1.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Second Messenger
    • Lipid Messengers
    • Immunology
    • Immune System Diseases
    • Allergens
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab85227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rabbit
Horse
Cow
Macaque monkey
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
  • Involvement in disease

    Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition.
  • Sequence similarities

    Belongs to the MAPEG family.
  • Domain

    The C-terminal part after residue 140 is mostly unstructured.
  • Cellular localization

    Nucleus membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P20292 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 100034178 Horse
    • Entrez Gene: 241 Human
    • Entrez Gene: 11690 Mouse
    • Entrez Gene: 100009413 Rabbit
    • Omim: 603700 Human
    • SwissProt: P30353 Horse
    • SwissProt: P20292 Human
    • SwissProt: P30355 Mouse
    • SwissProt: P30357 Rabbit
    • Unigene: 507658 Human
    • Unigene: 19844 Mouse
    see all
  • Alternative names

    • 5-lipoxygenase activating protein antibody
    • AL5AP_HUMAN antibody
    • ALOX 5AP antibody
    • ALOX5 AP antibody
    • ALOX5AP antibody
    • Arachidonate 5 lipoxygenase activating protein antibody
    • Arachidonate 5-lipoxygenase-activating protein antibody
    • Five lipoxygenase activating protein antibody
    • FLAP antibody
    • MK 886 binding protein antibody
    • MK-886-binding protein antibody
    see all

Images

  • Western blot - Anti-FLAP antibody (ab85227)
    Western blot - Anti-FLAP antibody (ab85227)
    All lanes : Anti-FLAP antibody (ab85227) at 1 µg/ml

    Lane 1 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa
    Additional bands at: 175 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab85227)
    Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab85227)
    ICC/IF image of ab85227 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab85227? Please let us know so that we can cite the reference in this datasheet.

ab85227 has been referenced in 8 publications.

  • Werner M  et al. Targeting biosynthetic networks of the proinflammatory and proresolving lipid metabolome. FASEB J 33:6140-6153 (2019). PubMed: 30735438
  • Rao Z  et al. Vacuolar (H+)-ATPase Critically Regulates Specialized Proresolving Mediator Pathways in Human M2-like Monocyte-Derived Macrophages and Has a Crucial Role in Resolution of Inflammation. J Immunol 203:1031-1043 (2019). PubMed: 31300512
  • Oddi S  et al. The anti-inflammatory agent bindarit acts as a modulator of fatty acid-binding protein 4 in human monocytic cells. Sci Rep 9:15155 (2019). PubMed: 31641194
  • Pein H  et al. Endogenous metabolites of vitamin E limit inflammation by targeting 5-lipoxygenase. Nat Commun 9:3834 (2018). PubMed: 30237488
  • Werz O  et al. Human macrophages differentially produce specific resolvin or leukotriene signals that depend on bacterial pathogenicity. Nat Commun 9:59 (2018). WB, IF ; Human . PubMed: 29302056
  • Garscha U  et al. Pharmacological profile and efficiency in vivo of diflapolin, the first dual inhibitor of 5-lipoxygenase-activating protein and soluble epoxide hydrolase. Sci Rep 7:9398 (2017). ICC/IF . PubMed: 28839250
  • Pace S  et al. Androgen-mediated sex bias impairs efficiency of leukotriene biosynthesis inhibitors in males. J Clin Invest 127:3167-3176 (2017). PubMed: 28737505
  • Garscha U  et al. BRP-187: A potent inhibitor of leukotriene biosynthesis that acts through impeding the dynamic 5-lipoxygenase/5-lipoxygenase-activating protein (FLAP) complex assembly. Biochem Pharmacol 119:17-26 (2016). PubMed: 27592027

Customer reviews and Q&As

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Western blot abreview for Anti-FLAP antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse bone marrow derived mast cells)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
1.5e+006 cells
Specification
mouse bone marrow derived mast cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Read More

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Submitted Jun 09 2016

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