Overview

  • Product name
  • Description
    Rabbit polyclonal to FLASH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    SERFQQLMKLFEKSKC

    , corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.)

  • Positive control
    • HeLa cell lysate An approximate 220 kDa band can be detected.
  • General notes


    A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    Affinity purified
  • Primary antibody notes
    A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes to discrete nuclear foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA387232 antibody
    • C8AP2_HUMAN antibody
    • CASP 8 associated protein 2 antibody
    • CASP8 associated protein 2 antibody
    • CASP8-associated protein 2 antibody
    • CASP8AP2 antibody
    • Caspase 8 associated protein 2 antibody
    • CED 4 antibody
    • CED4 antibody
    • D4Ertd659e antibody
    • FLASH homolog antibody
    • FLASH homolog RIP 25 antibody
    • FLASH homolog RIP25 antibody
    • FLICE associated huge antibody
    • FLICE associated huge protein antibody
    • FLICE-associated huge protein antibody
    • FLJ11208 antibody
    • KIAA1315 antibody
    • mKIAA1315 antibody
    • OTTMUSP00000005125 antibody
    • RIP 25 antibody
    • RIP25 antibody
    • RP23-69G24.1 antibody
    see all

Images

  • Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate

    Observed band size: 220 kDa (why is the actual band size different from the predicted?)

  • ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris-acetate)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up