Product nameFlavin Adenine Dinucleotide (FAD) Assay Kit
Sample typeSerum, Plasma, Cell Lysate, Cell culture media, Tissue Lysate
Assay typeEnzyme activity
Sensitivity< 1 nmol/well
Species reactivityReacts with: Mammals, Other species
Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710) is an assay where FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence. FAD can be detected by either colorimetric (OD=570 nm) or fluorometric (Ex/Em=535/587 nm) methods. The kit provides a rapid, simple, ultra-sensitive, and reliable test suitable for high throughput assay of FAD. The lower limit of detection is less than 1 nM FAD.
FAD assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader until absorbance reaches 1.8 at 570 nm (or stop before then)
Flavin Adenine Dinucleotide (FAD) is a redox cofactor which plays an important role in metabolism. FAD exists in different redox states and cycles between FAD, FADH and FADH2. The primary sources of reduced FAD in eukaryotic metabolism are the citric acid cycle and the beta oxidation reaction pathways.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests FAD Assay Buffer 1 x 25ml FAD Enzyme Mix (lyophilized) 1 vial FAD Standard (1 nmol, lyophilized) 1 vial OxiRed Probe (in DMSO) 1 x 200µl Stop Solution 1 x 1.2ml
ab204710 has been referenced in 1 publication.
- Nagano T et al. d-amino acid oxidase promotes cellular senescence via the production of reactive oxygen species. Life Sci Alliance 2:N/A (2019). PubMed: 30659069