Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)

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Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)
  • Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)

Key features and details

  • Mouse monoclonal [DM4/4/D6/5] to Flavivirus E-glycoprotein
  • Suitable for: ICC/IF
  • Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, West Nile virus, Zika virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5]
    See all Flavivirus E-glycoprotein primary antibodies

  • Description

    Mouse monoclonal [DM4/4/D6/5] to Flavivirus E-glycoprotein

  • Host species

    Mouse

  • Specificity

    This monoclonal antibody reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.

  • Tested applications

    Suitable for: ICC/IFmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, West Nile virus, Zika virus

  • Immunogen

    Recombinant full length protein corresponding to Dengue Virus 1 Flavivirus E-glycoprotein. DENV E (aa 281-775) and prM (aa 115-280) eluted from SDS-PAGE gels.
    Database link: P27909

  • Epitope

    This monoclonal antibody reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.

  • Positive control

    • Sandwich ELISA: Zika infected C6/36 cells; ICC/IF: DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Proclin 150
    Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    DM4/4/D6/5

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance

      The Flaviviridae are a family of viruses that are primarily spread through arthropod vectors (mainly ticks and mosquitoes). Flaviviridae have monopartite, linear, single-stranded RNA genomes of positive polarity, 9.6- to 12.3-kilobase in length with enveloped and spherical virus particles, about 40-60 nm in diameter.

    • Database links

      • Alternative names

        • Flavivirus envelope glycoprotein antibody

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)
        Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)

        Immunofluorescent analysis of DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214332 or an antibody control.

      • Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)
        Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [DM4/4/D6/5] (ab214332)

        Immunofluorescent analysis of DENV1 and DENV2 infected cells and not infected cells labeling Flavivirus E-glycoprotein using ab214332 or an antibody control.

      References (0)

      Publishing research using ab214332? Please let us know so that we can cite the reference in this datasheet.

      ab214332 has not yet been referenced specifically in any publications.

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