Key features and details
- Mouse monoclonal [HF8-1/C1-5/6/D2] to Flavivirus E-glycoprotein
- Suitable for: WB, ICC/IF
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
Product nameAnti-Flavivirus E-glycoprotein antibody [HF8-1/C1-5/6/D2]
See all Flavivirus E-glycoprotein primary antibodies
DescriptionMouse monoclonal [HF8-1/C1-5/6/D2] to Flavivirus E-glycoprotein
SpecificityThis monoclonal antibody reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
Recombinant full length protein corresponding to Dengue Virus 1 Flavivirus E-glycoprotein aa 281-775.
MRCVGIGNRDFVEGLSGATWVDVVLEHGSCVTTMAKNKPTLDIELLKTEV TNPAVLRKLC IEAKISNTTTDSRCPTQGEATLVEEQDANFVCRRTFVD RGWGNGCGLFGKGSLLTCAKFK CVTKLEGKIVQYENLKYSVIVTVHTG DQHQVGNETTEHGTIATITPQAPTSEIQLTDYGA LTLDCSPRTGLDFN EMVLLTMKEKSWLVHKQWFLDLPLPWTSGASTSQETWNRQDLLVTF KT AHAKKQEVVVLGSQEGAMHTALTGATEIQTSGTTTIFAGHLKCRLKMDKL TLKGTSYV MCTGSFKLEKEVAETQHGTVLVQVKYEGTDAPCKIPFSTQ DEKGVTQNGRLITANPIVTD KEKPVNIETEPPFGESYIVVGAGEKALK LSWFKKGSSIGKMFEATARGARRMAILGDTAW DFGSIGGVFTSVGKLV HQVFGTAYGVLFSGVSWTMKIGIGILLTWLGLNSRSTSLSMTCI AVGM VTLYLGVMVQA
Database link: P27909
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab214331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceThe Flaviviridae are a family of viruses that are primarily spread through arthropod vectors (mainly ticks and mosquitoes). Flaviviridae have monopartite, linear, single-stranded RNA genomes of positive polarity, 9.6- to 12.3-kilobase in length with enveloped and spherical virus particles, about 40-60 nm in diameter.
- Flavivirus envelope glycoprotein antibody
Immunofluorescent analysis of DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214331 or an antibody control.
Immunofluorescent analysis of DENV1, DENV2 infected cells and uninfected cells labeling Flavivirus E-glycoprotein using ab214331 or an antibody control.
All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-5/6/D2] (ab214331)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : C6/36 cell lysate control (uninfected)
ab214331 has not yet been referenced specifically in any publications.