Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-5/6/D2] (ab214331)
Key features and details
- Mouse monoclonal [HF8-1/C1-5/6/D2] to Flavivirus E-glycoprotein
- Suitable for: WB, ICC/IF
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
Overview
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Product name
Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-5/6/D2]
See all Flavivirus E-glycoprotein primary antibodies -
Description
Mouse monoclonal [HF8-1/C1-5/6/D2] to Flavivirus E-glycoprotein -
Host species
Mouse -
Specificity
This monoclonal antibody reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus -
Immunogen
Recombinant full length protein corresponding to Dengue virus 1 Flavivirus E-glycoprotein aa 250-800.
Database link: P27909 -
Positive control
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HF8-1/C1-5/6/D2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
The Flaviviridae are a family of viruses that are primarily spread through arthropod vectors (mainly ticks and mosquitoes). Flaviviridae have monopartite, linear, single-stranded RNA genomes of positive polarity, 9.6- to 12.3-kilobase in length with enveloped and spherical virus particles, about 40-60 nm in diameter. -
Database links
- SwissProt: P27909 Dengue virus 1
- SwissProt: P29990 Dengue virus 2
- SwissProt: P29991 Dengue virus 2
- SwissProt: Q6YMS4 Dengue virus 3
- SwissProt: Q2YHF0 Dengue virus 4
- SwissProt: P14403 Japanese encephalitis virus
- SwissProt: P06935 West Nile virus
- SwissProt: Q32ZE1 Zika virus
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Alternative names
- Flavivirus envelope glycoprotein antibody
Images
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Immunofluorescent analysis of DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214331 or an antibody control.
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Immunofluorescent analysis of DENV1, DENV2 infected cells and uninfected cells labeling Flavivirus E-glycoprotein using ab214331 or an antibody control.
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All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-5/6/D2] (ab214331)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : C6/36 cell lysate control (uninfected)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab214331 has not yet been referenced specifically in any publications.