Overview

  • Product name

    Anti-Flavivirus E-glycoprotein antibody [HF8-2/B2-2/E10]
    See all Flavivirus E-glycoprotein primary antibodies
  • Description

    Mouse monoclonal [HF8-2/B2-2/E10] to Flavivirus E-glycoprotein
  • Host species

    Mouse
  • Specificity

    This monoclonal antibody reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV.
  • Tested applications

    Suitable for: WB, Sandwich ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
  • Immunogen

    Recombinant full length protein corresponding to Dengue Virus 1 Flavivirus E-glycoprotein aa 281-775.
    Database link: P27909

  • Positive control

    • WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells. Sandwich ELISA: DENV1, DENV2 (NGC), DENV2 (16681) DENV3, DENV4, JEV.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Proclin
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    HF8-2/B2-2/E10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Immunofluorescent analysis of DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214333 or an antibody control.

  • Immunofluorescent analysis of DENV1, DENV2 infected and uninfected cells labeling Flavivirus E-glycoprotein using ab214333 or an antibody control.

  • All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-2/B2-2/E10] (ab214333)

    Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
    Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
    Lane 3 : DENV3 (H89) infected C6/36 cell lysate
    Lane 4 : DENV4 (H241) infected C6/36 cell lysate
    Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
    Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
    Lane 7 : YFV (17D) infected C6/36 cell lysate
    Lane 8 : WNV (E101) infected C6/36 cell lysate
    Lane 9 : C6/36 cell lysate uninfected control
  • Sandwich ELISA analysis of Flavivirus E-glycoprotein expression in DENV1, DENV2 (NGC), DENV2 (16681) DENV3, DENV4, JEV and control using ab214333 as capture antibody.

References

ab214333 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214333.
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